使用丹磺酰肼荧光衍生化的微高效液相色谱法测定前列腺素E2及主要前列腺素E代谢物
Determination of prostaglandin E2 and the main prostaglandin E metabolite by micro high-performance liquid chromatography using fluorescence derivatization with dansyl hydrazine.
作者信息
Yamada K, Onodera M, Aizawa Y
出版信息
J Pharmacol Methods. 1983 Apr;9(2):93-100. doi: 10.1016/0160-5402(83)90001-3.
A micro high-performance liquid chromatographic method for the determination of prostaglandin E2(PGE2) and the main prostaglandin E metabolite (PGEMT) in rat organs and human urine has been developed. Prednisolone was added to the sample as the internal standard. The isolated PG's and the internal standard were subjected to reaction with dansyl hydrazine, separated by liquid chromatography on a mu Fine pack SIL C18 and determined fluorimetrically. The standard deviation of the method was +/- 6.5% for PGE2, and +/- 8.4% for PGEMT. The detection and simultaneous quantitation of less than 10 ng of PGE2 and PGEMT is possible. The proposed method is suitable for the pharmacological analysis of PGE2 and PGEMT in various rat tissues and human urine.
已开发出一种用于测定大鼠器官和人尿中前列腺素E2（PGE2）及主要前列腺素E代谢物（PGEMT）的微型高效液相色谱法。将泼尼松龙作为内标加入样品中。分离出的前列腺素和内标与丹磺酰肼反应，在μFine pack SIL C18上进行液相色谱分离，并用荧光法测定。该方法对PGE2的标准偏差为±6.5%，对PGEMT为±8.4%。能够检测并同时定量低至10 ng的PGE2和PGEMT。所提出的方法适用于各种大鼠组织和人尿中PGE2和PGEMT的药理分析。
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