Jung G, Férard G
Clin Chem. 1978 Jun;24(6):873-6.
We describe an automated enzymic method for ethanol determination with a centrifugal analyzer (the GEMSAEC) by measuring the rate of the reaction catalyzed by alcohol dehydrogenase and coupled to aldehyde dehydrogenase. The detection limits, reproducibility, and accuracy of the method have been evaluated. It can be applied to whole blood or plasma, with or without previous deproteinization. Our results, compared with those by an automated alcohol dehydrogenase method in the presence of semicarbazide, show an improved linearity, sensitivity, and rapidity of determination.
我们描述了一种使用离心分析仪（GEMSAEC）通过测量酒精脱氢酶催化并与醛脱氢酶偶联的反应速率来测定乙醇的自动化酶法。已对该方法的检测限、重现性和准确性进行了评估。它可应用于全血或血浆，无论是否预先进行脱蛋白处理。与在存在氨基脲的情况下使用自动化酒精脱氢酶法的结果相比，我们的结果显示出测定的线性、灵敏度和速度均有所提高。
