Higashida H, Kano-Tanaka K, Natsuume-Sakai S, Sudo K, Fukami H, Nakagawa Y, Miki N
Int J Cancer. 1983 Jun 15;31(6):797-802. doi: 10.1002/ijc.2910310621.
Addition to the culture medium of prostaglandin (PG) D2 resulted in the degeneration in a dose- and time-dependent manner of N18TG-2 cells cloned from mouse neuroblastoma. The ED50 for PGD2-induced cytotoxicity was about 10 microM. The degenerative changes were irreversible when the cells were exposed for more than 10 h. Scanning and transmission electron microscopic examination revealed that treatment with PGD2 resulted in appearance of numerous blebs of various sizes along the cell surface and also in destruction of surface membrane and of cytoplasmic organelles. Tumor weight of N18TG-2 neuroblastoma inoculated subcutaneously on the backs of A/J mice was about 35-70% less than that of controls after 14 days of single daily i.p. or s.c. injections of 0.5-1 mg/kg of PGD2. The results indicate that PGD2 has growth-inhibitory effects on mouse neuroblastoma cells in vitro and in vivo.
向培养基中添加前列腺素（PG）D2会导致从小鼠神经母细胞瘤克隆的N18TG - 2细胞以剂量和时间依赖性方式发生退化。PGD2诱导细胞毒性的半数有效剂量（ED50）约为10微摩尔。当细胞暴露超过10小时时，退化性变化是不可逆的。扫描电子显微镜和透射电子显微镜检查显示，用PGD2处理会导致细胞表面出现许多大小各异的泡状突起，同时还会破坏表面膜和细胞质细胞器。在A/J小鼠背部皮下接种N18TG - 2神经母细胞瘤后，每天腹腔注射或皮下注射0.5 - 1毫克/千克的PGD2，持续14天，肿瘤重量比对照组减少约35 - 70%。结果表明，PGD2在体外和体内对小鼠神经母细胞瘤细胞均具有生长抑制作用。
