Muta M, Yorishima M, Nagai N, Nogawa T, Hiura M, Fujiwara A
Nihon Sanka Fujinka Gakkai Zasshi. 1983 May;35(5):661-4.
A case of a virilizing ovarian Leydig cell tumor in a 38 year-old woman with a markedly elevated plasma testosterone level was investigated using light microscopic dry-mounting radioautography and electron microscopic cytochemistry. Following total abdominal hysterectomy and bilateral salpingo-oophorectomy, the plasma testosterone level fell abruptly. Light microscopic dry-mounting radioautography for 3H-cholesterol showed silver grains mainly localized over the cytoplasm of the tumor cells. In the Leydig cell tumor there were three different cell types: Fibroblast-like cells, Leydig cells (steroid-secreting cells) and transitional cells (partially or incompletely differentiated Leydig cells). Reaction products for 3 beta-hydroxysteroid dehydrogenase activity were localized on tubular or lamellar cristae and inner membranes of the mitochondria, and on the membranes of smooth endoplasmic reticulum in the transitional cell as well as in the Leydig cell. From these facts, it is suggested that the Leydig cell tumor is derived from the fibroblast-like cell and the Leydig cell, already has a steroidogenic activity in secreting testosterone.
对一名38岁血浆睾酮水平显著升高的女性的男性化卵巢间质细胞瘤病例，采用光学显微镜干片放射自显影术和电子显微镜细胞化学进行了研究。全腹子宫切除和双侧输卵管卵巢切除术后，血浆睾酮水平急剧下降。用³H-胆固醇进行光学显微镜干片放射自显影显示，银颗粒主要位于肿瘤细胞的细胞质上。间质细胞瘤中有三种不同的细胞类型：成纤维细胞样细胞、间质细胞（类固醇分泌细胞）和过渡细胞（部分或不完全分化的间质细胞）。³β-羟基类固醇脱氢酶活性的反应产物定位于过渡细胞和间质细胞中线粒体的管状或板层嵴及内膜，以及滑面内质网的膜上。从这些事实推测，间质细胞瘤起源于成纤维细胞样细胞和间质细胞，已经具有分泌睾酮的类固醇生成活性。
