Okahara T, Imanishi M, Yamamoto K
Prostaglandins. 1983 Mar;25(3):373-83. doi: 10.1016/0090-6980(83)90040-0.
The release of prostaglandin(PG) and thromboxane(TX) was examined in the six different areas of the normal dog kidney, i.e., renal arterial and venous strips(RA and RV), superficial and deep cortical slices(SC and DC) and outer and inner medullary slices(OM and IM). These tissues were incubated in Krebs-bicarbonate buffer(pH 7.4, 37 degrees C), and the released PGE2, PGF2 alpha, 6-keto-PGF1 alpha and TXB2(as stable metabolites of PGI2 and TXA2, respectively) were determined by radioimmunoassay. In RA, RV, SC and DC, 6-keto-PGF1 alpha was predominant, however, there were no quantitative differences between RA and RV, or SC and DC. The release of 6-keto-PGF1 alpha reached a maximum in IM, similar to findings on the release of PGE2 and PGF2 alpha. The release of TXB2 was uniform in OM and IM. The amount of PGE2, PGF2 alpha, 6-keto-PGF1 alpha and TXB2 released from IM was 2800, 400, 60 and 50 times higher, respectively, than the extent of the release from the cortical slices. These results suggest that PGI2 as well as PGE2 and PGF2 alpha, may be involved in renal PG, and that TXA2 is biosynthesized in the normal dog kidney.
研究了正常犬肾六个不同区域前列腺素（PG）和血栓素（TX）的释放情况，即肾动脉和静脉条带（RA和RV）、浅层和深层皮质切片（SC和DC）以及外层和内层髓质切片（OM和IM）。将这些组织置于Krebs - 碳酸氢盐缓冲液（pH 7.4，37℃）中孵育，通过放射免疫测定法测定释放的前列腺素E2（PGE2）、前列腺素F2α（PGF2α）、6 - 酮 - 前列腺素F1α（6 - keto - PGF1α）和血栓素B2（TXB2，分别为前列环素（PGI2）和血栓素A2（TXA2）的稳定代谢产物）。在RA、RV、SC和DC中，6 - keto - PGF1α占主导地位，然而，RA与RV之间或SC与DC之间没有定量差异。6 - keto - PGF1α的释放在IM中达到最大值，这与PGE2和PGF2α释放的研究结果相似。TXB2的释放在OM和IM中是均匀的。从IM释放的PGE2、PGF2α、6 - keto - PGF1α和TXB2的量分别比从皮质切片释放的量高2800倍、400倍、60倍和50倍。这些结果表明，PGI2以及PGE2和PGF2α可能参与肾PG的调节，并且TXA2在正常犬肾中生物合成。
