一种用于测定尿酸和次黄嘌呤/黄嘌呤的两步酶法。
A two-stage enzymatic method for determination of uric acid and hypoxanthine/xanthine.
作者信息
Cunningham S K, Keaveny T V
出版信息
Clin Chim Acta. 1978 Jun;86(2):217-21. doi: 10.1016/0009-8981(78)90135-3.
PMID:657544
Abstract
A method is described by which plasma or serum concentrations of uric acid and hypoxanthine/xanthine may be determined in a two-step sequential method. The principle of the method is that hydrogen peroxide, liberated by the action of uricase and xanthine oxidase on their substrates, is coupled to the production of a coloured product.
摘要
本文描述了一种方法，通过该方法可采用两步顺序法测定血浆或血清中尿酸以及次黄嘌呤/黄嘌呤的浓度。该方法的原理是，尿酸酶和黄嘌呤氧化酶作用于其底物所释放的过氧化氢，与有色产物的生成相偶联。
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