Bretzlaff K N, Hill A, Ott R S
Am J Vet Res. 1983 Jun;44(6):1162-4.
On day 10 of each estrous cycle, 19 cycling does were given (IM) 1 of 3 dosage levels of prostaglandin F2 alpha (PGF2 alpha) or saline solution. After injections, goats were observed twice daily for behavioral estrus, and serum concentrations of progesterone (P4) were determined from daily blood collections to measure response to treatment. Every dose of PGF2 alpha (0.0165 mg/kg of body weight, 0.0275 mg/kg, and 0.0385 mg/kg) was administered during 20 estrous cycles, and 0.25 ml of saline solution was administered during 12 estrous cycles. Fourteen of 20, 15 of 20, 20 of 20, and 0 of 12 does exhibited signs of behavioral estrus within 96 hours of injections of 0.0165 mg of PGF2 alpha/kg, 0.0275 mg of PGF2 alpha/kg, 0.0385 mg of PGF2 alpha/kg, and saline solution, respectively. A decline in mean concentrations of peripheral P4 from 3.6, 3.8, 4.4, and 4.3 ng/ml to less than 1 ng/ml within 24 hours was observed in 15 of 20, 16 of 20, 20 of 20, and 0 of 10 does after injections of 0.0165 mg of PGF2 alpha/kg, 0.0275 mg of PGF2 alpha/kg, 0.0385 mg of PGF2 alpha/kg, and saline solution, respectively. The dosage level of 0.0385 mg of PGF2 alpha/kg (1.75 mg of PGF2 alpha/45.36 kg) was effective for induction of estrus in 100% of the does.
在每个发情周期的第10天，给19只处于发情期的母羊肌肉注射三种剂量水平之一的前列腺素F2α（PGF2α）或生理盐水。注射后，每天观察母羊两次，记录行为发情情况，并通过每天采集血液测定血清孕酮（P4）浓度，以评估治疗反应。每种剂量的PGF2α（0.0165毫克/千克体重、0.0275毫克/千克和0.0385毫克/千克）在20个发情周期中给药，0.25毫升生理盐水在12个发情周期中给药。在注射0.0165毫克PGF2α/千克、0.0275毫克PGF2α/千克、0.0385毫克PGF2α/千克和生理盐水后，分别有20只中的14只、20只中的15只、20只中的20只和12只中的0只在96小时内表现出行为发情迹象。在注射0.0165毫克PGF2α/千克、0.0275毫克PGF2α/千克、0.0385毫克PGF2α/千克和生理盐水后，分别有20只中的15只、20只中的16只、20只中的20只和10只中的0只在24小时内外周P4平均浓度从3.6、3.8、4.4和4.3纳克/毫升降至低于1纳克/毫升。0.0385毫克PGF2α/千克（1.75毫克PGF2α/45.36千克）的剂量水平对100%的母羊诱导发情有效。
