Legge M, Potter H C
N Z Med J. 1983 Aug 24;96(738):648-50.
Amniotic fluid acetylcholinesterase (AChe) activity was assayed in second trimester amniotic fluids by inhibition of non-specific cholinesterase using lysivane. Of the 196 samples analysed, 146 were from normal pregnancies; 11 (5.6%) from severe open neural tube defects; two from small open spina bifidas; seven from fetal chromosome abnormalities and two from severe skeletal dysplasias. In addition nine amniotic fluids were meconium stained and 19 (9.7%) had varying degrees of blood staining. Acetylcholinesterase values remained relatively constant from 13-21 weeks gestation in normal pregnancies and a cut-off limit of 3.2 U/l was set (mean + 2 SD). Heavily blood stained amniotic fluids were associated with AChe levels on or above the normal cut-off limit. Meconium staining caused a non-significant elevation of AChe. No significant elevation of AChe was demonstrated for fetal chromosome abnormalities, or severe skeletal dysplasias. Anencephaly and severe open spina bifida had significantly elevated AChe levels.
采用赖氨酯抑制非特异性胆碱酯酶的方法，对孕中期羊水的乙酰胆碱酯酶（AChe）活性进行检测。在分析的196份样本中，146份来自正常妊娠；11份（5.6%）来自严重开放性神经管缺陷；2份来自小型开放性脊柱裂；7份来自胎儿染色体异常；2份来自严重骨骼发育不良。此外，9份羊水被胎粪污染，19份（9.7%）有不同程度的血染。正常妊娠时，妊娠13 - 21周期间乙酰胆碱酯酶值相对恒定，设定临界值为3.2 U/l（均值 + 2倍标准差）。重度血染羊水的乙酰胆碱酯酶水平等于或高于正常临界值。胎粪污染导致乙酰胆碱酯酶轻度升高，但无统计学意义。胎儿染色体异常或严重骨骼发育不良未显示乙酰胆碱酯酶显著升高。无脑儿和严重开放性脊柱裂的乙酰胆碱酯酶水平显著升高。
