Tønnesen E, Mickley H, Grunnet N
Acta Anaesthesiol Scand. 1983 Jun;27(3):238-41. doi: 10.1111/j.1399-6576.1983.tb01943.x.
Natural killer (NK) cell activity of peripheral blood mononuclear cells was measured against K-562 target cells in a 51Cr release assay in eight patients undergoing total hip replacement surgery. Eight consecutive blood samples were taken from each patient. A significant increase of NK cell activity was observed after premedication with diazepam per os. The activity increased further during a combined anaesthesia (thiopentone + N2O + O2 + buprenorphene + pancuronium) and remained increased during surgery. Postoperatively, NK cell activity fell and remained depressed for a period of at least 5 days. The findings of this study indicate that premedication, anaesthesia and surgery cause a rapid and transient increase in NK cell activity, followed by a decline in activity postoperatively. The transient increase in activity may be explained by mobilization of natural killer cells from extravasal space, spleen or lymph nodes into the circulation. The clinical significance of the alterations in NK cell activity is unknown and needs further investigation.
在51Cr释放试验中，对8例行全髋关节置换手术的患者，测定其外周血单个核细胞针对K-562靶细胞的自然杀伤（NK）细胞活性。从每位患者采集连续8份血样。口服地西泮进行术前用药后，观察到NK细胞活性显著增加。在联合麻醉（硫喷妥钠+氧化亚氮+氧气+丁丙诺啡+泮库溴铵）期间活性进一步升高，并在手术过程中持续升高。术后，NK细胞活性下降，并至少持续5天处于抑制状态。本研究结果表明，术前用药、麻醉和手术会导致NK细胞活性迅速短暂升高，随后术后活性下降。活性的短暂升高可能是由于自然杀伤细胞从血管外间隙、脾脏或淋巴结动员进入循环所致。NK细胞活性改变的临床意义尚不清楚，需要进一步研究。
