离体大鼠肾小管中氟乙酸盐中毒的生化方面：α-酮戊二酸对糖异生抑制作用的可逆性

Biochemical aspects of fluoroacetate poisoning in isolated rat kidney tubules: reversibility of inhibition of gluconeogenesis by alpha-ketoglutarate.

作者信息

Wiedemann P, Szinicz L, Weger N

出版信息

Arch Toxicol Suppl. 1983;6:232-7. doi: 10.1007/978-3-642-69083-9_42.

Abstract

The effects of monofluoroacetate (FAC) on suspensions of isolated rat kidney tubules were investigated. FAC inhibited gluconeogenesis from lactate, pyruvate, fructose, dihydroxyacetone, alpha-ketoglutarate, and succinate. The gluconeogenesis from pyruvate, ketoglutarate, and lactate was less sensitive to FAC than that from other substrates. FAC also caused a decrease in oxygen consumption, hydroxybutyrate to acetoacetate ratio, alpha-ketoglutarate, ATP, and total adenine nucleotide content; the citrate content was increased. Addition of alpha-ketoglutarate, 5 mmol/l, caused a reversal of gluconeogenesis inhibition, an increase in ATP content, and a delay in citrate accumulation in isolated rat kidney tubules incubated with FAC.

摘要

研究了一氟乙酸（FAC）对分离的大鼠肾小管悬液的影响。FAC抑制了由乳酸、丙酮酸、果糖、二羟基丙酮、α-酮戊二酸和琥珀酸生成葡萄糖的过程。由丙酮酸、酮戊二酸和乳酸生成葡萄糖的过程对FAC的敏感性低于由其他底物生成葡萄糖的过程。FAC还导致氧消耗量、β-羟基丁酸与乙酰乙酸的比率、α-酮戊二酸、ATP和总腺嘌呤核苷酸含量降低；柠檬酸含量增加。添加5 mmol/l的α-酮戊二酸可使在FAC中孵育的分离大鼠肾小管中葡萄糖生成抑制作用逆转、ATP含量增加并延迟柠檬酸积累。