Stavem P, Kjaerheim A
Scand J Haematol. 1977 Feb;18(2):170-6. doi: 10.1111/j.1600-0609.1977.tb02087.x.
A few years ago a patients, T.L., with metastatic leiomyosarcoma found to have a serum and tumor factor which caused the granulomere of normal platelets to disappear as seen using light microscopy. The present report describes the effect on platelet granules of several different substances using various anticoagulants. Ultramicroscopy showed in all instances that the platelet stain preventing effect as seen in May-Grünwald & Giemsa stained films reflected a degranulation. Ultramicroscopy was little better than light microscopy in the differentiation between the mechanisms of the various degranulating substances. Different conditions such as variation in anticoagulant or combination of anticoagulants, storage at room temperature for 24h, heating to 56 degrees C for 1 h on the other hand were very useful methods for differentiating between the various degranulating substances. These methods failed, however, to differentiate between patient T.L.-serum and patient T.L.-tumour fraction SSS, probably because the active factor was the same in both.
几年前，一位患有转移性平滑肌肉瘤的患者T.L.，其血清和肿瘤因子可使正常血小板的颗粒在光学显微镜下消失。本报告描述了使用多种抗凝剂时几种不同物质对血小板颗粒的影响。超微显微镜检查在所有情况下均显示，在May-Grünwald和姬姆萨染色片中看到的血小板染色阻止效应反映了脱颗粒现象。在区分各种脱颗粒物质的机制方面，超微显微镜检查并不比光学显微镜好多少。另一方面，不同的条件，如抗凝剂的变化或抗凝剂的组合、在室温下储存24小时、加热至56摄氏度1小时，是区分各种脱颗粒物质的非常有用的方法。然而，这些方法未能区分患者T.L.血清和患者T.L.肿瘤组分SSS，可能是因为两者中的活性因子相同。
