Wilken M, Plesner T, Geisler C, Bjerrum O J
Acta Pathol Microbiol Immunol Scand C. 1983 Aug;91(4):237-44.
Crossed immunoelectrophoresis was used to study the antigens of chronic lymphocytic leukemia lymphocytes. A reference pattern was obtained and 20 samples from 18 patients were compared with this pattern. Extensive variation in the expression of individual antigens was observed. The antigens in the reference pattern were further characterized by modifications of crossed immunoelectrophoresis, viz. labelling of intact lymphocytes by lactoperoxidase-catalyzed iodination, affinity crossed immunoelectrophoresis with phenyl-Sepharose and Lentil lectin-Sepharose, charge-shift crossed immunoelectrophoresis and postelectrophoretic incubation in (125I) Lentil lectin. Six antigens were identified as surface membrane glycoproteins, 5 as cytoplasmic proteins while 5 could not be classified. Two antigens were finally identified as HLA-ABC and HLA-DR by application of small amounts of monospecific rabbit antiserum and monoclonal antibody, respectively.
采用交叉免疫电泳法研究慢性淋巴细胞白血病淋巴细胞的抗原。获得了一个参考图谱，并将18例患者的20份样本与该图谱进行了比较。观察到各个抗原表达存在广泛差异。通过交叉免疫电泳的改进方法对参考图谱中的抗原进行了进一步表征，即通过乳过氧化物酶催化碘化对完整淋巴细胞进行标记、用苯基琼脂糖和扁豆凝集素琼脂糖进行亲和交叉免疫电泳、电荷转移交叉免疫电泳以及在（125I）扁豆凝集素中进行电泳后孵育。鉴定出6种抗原为表面膜糖蛋白，5种为细胞质蛋白，还有5种无法分类。最后分别应用少量单特异性兔抗血清和单克隆抗体将两种抗原鉴定为HLA - ABC和HLA - DR。
