Acker H, Holtermann G, Carlsson J, Nederman T
Adv Exp Med Biol. 1983;159:445-62. doi: 10.1007/978-1-4684-7790-0_38.
Different types of oxygen microelectrodes have been tested in measurements on cellular spheroids. The shape of the oxygen gradients varied strongly depending on size and type of the spheroids. No significant differences in the results were obtained when different types of electrodes were applied. All measurements were made in a perfusion chamber. The shape of the gradients did not vary with time in the perfusion chamber. The reproducibility was found good in repeated measurements using the same spheroid. No mechanical or chemical disturbances were seen during the penetration of the spheroids. Changes in the medium flow rate through the chamber did not drastically change the shape of the oxygen gradients. Almost no convection could be seen at the bottom of the chamber close to the spheroids. The composition of the medium was found to be of importance. Lock's solution containing glucose was found to be satisfactory. The potential signals in the double barrel electrodes allowed an accurate determination of the position when the electrode hit the spheroid surface. The information gained from microelectrode measurements in spheroids might be valuable for the understanding of effects of new tumor treatment modalities in which hypoxic cell sensitizers or high LET radiation are utilized.
不同类型的氧微电极已在细胞球体测量中进行了测试。氧梯度的形状因球体的大小和类型而有很大差异。使用不同类型的电极时，结果没有显著差异。所有测量均在灌注室中进行。在灌注室内，梯度的形状不会随时间变化。使用同一个球体进行重复测量时，发现重现性良好。在球体穿刺过程中未观察到机械或化学干扰。通过灌注室的介质流速变化不会显著改变氧梯度的形状。在靠近球体的灌注室底部几乎看不到对流。发现介质的成分很重要。含有葡萄糖的洛克氏溶液被认为是令人满意的。双管电极中的电位信号可以在电极接触球体表面时准确确定位置。从球体微电极测量中获得的信息对于理解使用乏氧细胞增敏剂或高传能线密度辐射的新肿瘤治疗方式的效果可能具有重要价值。
