对过氧化物酶活性以及激素17β-雌二醇与大鼠唾液腺特异性结合的影响。

Effect on peroxidase activity and specific binding of the hormone 17 beta-oestradiol and rat salivary glands.

作者信息

Laine M, Tenovuo J

出版信息

Arch Oral Biol. 1983;28(9):847-52. doi: 10.1016/0003-9969(83)90042-0.

Abstract

The effect was studied on immature and adult female rats. Oestradiol, given either once (10 micrograms) or three times (3 X 10 micrograms) subcutaneously significantly retarded the total weight gain of immature rats whereas, at the same time, the weight of the uteri increased several fold. The weights of neither salivary nor lacrimal glands were dependent on oestradiol. The activity of the peroxidase enzyme, a marker of oestrogen-responsive tissues, increased significantly in the rat uteri but had no effect on lacrimal peroxidase. The levels of rat salivary peroxidase increased during oestradiol administration with considerable differences between various glands. The increase in peroxidase activities could not be explained by changes in the total protein content but seemed to be specific for this enzyme. Experiments using [3H]-oestradiol indicated specific binding to uterus, parotid and submandibular glands. It is concluded that rat salivary glands are oestrogen-responsive.

摘要

对未成年和成年雌性大鼠进行了该效应的研究。皮下注射一次雌二醇（10微克）或三次（3×10微克）可显著延缓未成年大鼠的总体重增加，而与此同时，子宫重量增加了数倍。唾液腺和泪腺的重量均不依赖于雌二醇。过氧化物酶（雌激素反应性组织的标志物）的活性在大鼠子宫中显著增加，但对泪腺过氧化物酶没有影响。在给予雌二醇期间，大鼠唾液过氧化物酶水平升高，不同腺体之间存在显著差异。过氧化物酶活性的增加不能用总蛋白含量的变化来解释，似乎是该酶特有的。使用[3H] - 雌二醇的实验表明其与子宫、腮腺和颌下腺有特异性结合。结论是大鼠唾液腺对雌激素有反应。