Stefanova T, Mutafchieva M
Eksp Med Morfol. 1978;17(2):104-8.
The authors describe a method for determination of estrogens in urine of nonpregnant women and men. The method includes enzymic hydrolysis of urine, gel filtration of Sefadex G25, extraction with organic solvents and modifies Kober reaction with spectrofluorometric determination. The technique of the method is not complex, the usage of reagents is small and is suitable for everyday work.
作者描述了一种测定非妊娠女性和男性尿液中雌激素的方法。该方法包括尿液的酶水解、Sephadex G25凝胶过滤、用有机溶剂萃取以及用荧光分光光度法测定改良的科伯反应。该方法的技术并不复杂,试剂用量少,适用于日常工作。