Orosz M, Csapó I, Varga B
Prostaglandins. 1983 Aug;26(2):165-73. doi: 10.1016/0090-6980(83)90085-0.
Dioestrous and pregnant, or ovariectomized hamsters treated with sunflower oil, oestradiol or progesterone were anesthetized with pentobarbital and the arterioles of the cheek pouch membrane were prepared for microcirculatory study. Prostaglandin E2 (PGE2) or noradrenaline (NA) were topically applied and changes of the arteriolar diameter were measured on the screen of a closed-circuit television. PGE2 induced arteriolar dilatation in dioestrous or ovariectomized hamsters, but induced vasoconstriction in pregnant or in oestrogen-treated animals. Vasoconstriction induced by PGE2 in oestrogen-treated animals disappeared after administration of alpha-adrenergic blockers. In this situation, PGE2 induced vasodilatation once again. NA elicited arteriolar constriction in each experimental group. In ovariectomized hamsters treated with oestrogen the constriction was more pronounced than in progesterone treated animals. In pregnant animals it was significantly greater on day 14 than in dioestrous animals. Progesterone treatment blunted the vascular effect of both PGE2 and NA. It was concluded that the reverse effect of PGE2 and the increased sensitivity to NA induced by high oestradiol levels may have roles in the regulation of local blood flow during the ovarian cycle and pregnancy.
处于动情后期和妊娠期的仓鼠，或用向日葵油、雌二醇或孕酮处理过的去卵巢仓鼠，用戊巴比妥麻醉后，准备对颊囊膜的小动脉进行微循环研究。局部应用前列腺素E2（PGE2）或去甲肾上腺素（NA），并在闭路电视屏幕上测量小动脉直径的变化。PGE2可使动情后期或去卵巢仓鼠的小动脉扩张，但可使妊娠或经雌激素处理的动物的小动脉收缩。在给予α-肾上腺素能阻滞剂后，PGE2在经雌激素处理的动物中诱导的血管收缩消失。在这种情况下，PGE2再次诱导血管扩张。NA在每个实验组中均引起小动脉收缩。在用雌激素处理的去卵巢仓鼠中，收缩比用孕酮处理的动物更明显。在妊娠动物中，第14天时的收缩明显大于动情后期动物。孕酮处理减弱了PGE2和NA的血管效应。得出的结论是，PGE2的反向作用以及高雌二醇水平诱导的对NA敏感性增加可能在卵巢周期和妊娠期间局部血流的调节中起作用。
