Hibbin J A, McCarthy D M, Goldman J M
Scand J Haematol. 1983 Nov;31(5):454-60. doi: 10.1111/j.1600-0609.1983.tb01543.x.
We assayed the number of multilineage myeloid progenitor cells (CFU-GEMM) in the blood and marrow of patients with newly diagnosed chronic granulocytic leukaemia (CGL). The mean number of CFU-GEMM in the blood was increased 600-fold and CFU-GEMM in the marrow was doubled in the CGL patients compared with normal. A complement-fixing monoclonal antibody with HLA-DR specificity inhibited the proliferation of CFU-GEMM from CGL blood to a greater extent than that of comparable cells in normal marrow. Using a hydroxyurea 'suicide' method we found that the proportion of CFU-GEMM in proliferative cycle was higher in CGL blood than in normal marrow. We conclude that (1) CFU-GEMM numbers are greatly increased in the blood of patients with CGL, (2) CFU-GEMM express HLA-DR antigens on their surface, and (3) the apparently increased expression of the antigen on CFU-GEMM from CGL blood in comparison with CFU-GEMM from normal marrow may parallel the relatively higher proportion of CGL CFU-GEMM in cell cycle.
我们检测了新诊断的慢性粒细胞白血病（CGL）患者血液和骨髓中多谱系髓系祖细胞（CFU - GEMM）的数量。与正常人相比，CGL患者血液中CFU - GEMM的平均数量增加了600倍，骨髓中的CFU - GEMM数量翻倍。一种具有HLA - DR特异性的补体结合单克隆抗体对CGL血液中CFU - GEMM增殖的抑制程度比对正常骨髓中类似细胞的抑制程度更大。使用羟基脲“自杀”方法，我们发现CGL血液中处于增殖周期的CFU - GEMM比例高于正常骨髓。我们得出结论：（1）CGL患者血液中CFU - GEMM数量大幅增加；（2）CFU - GEMM在其表面表达HLA - DR抗原；（3）与正常骨髓中的CFU - GEMM相比，CGL血液中CFU - GEMM上该抗原的明显增加表达可能与CGL的CFU - GEMM在细胞周期中相对较高的比例平行。
