Andersson B, Leffler H, Magnusson G, Svanborg Edén C
Scand J Infect Dis Suppl. 1983;39:45-7.
Adherence of bacteria to mucosal surfaces is an early step in colonization and infection. Streptococcus pneumoniae colonizes the nasopharynx and causes localized and systemic disease. The higher adhesion capacity of strains isolated from patients with otitis as compared to strains from sepsis or meningitis patients indicates that binding to the nasopharyngeal mucosa is important in localized but not in invasive infections. The biochemical basis of the pneumococcal binding to epithelial cells has been investigated. Human nasopharyngeal epithelial cells were mixed with pneumococci, and adherence was determined microscopically. Inhibition of adherence with analogues of epithelial cell receptors was tested by preincubation of pneumococci before the addition of the epithelial cells. Competitive inhibition with glycoconjugates, representing receptor structures on the epithelial cell surface, showed receptor function of saccharides containing the disaccharide GlcNAcbeta1 leads to 3Galbeta-.
细菌黏附于黏膜表面是定植和感染的早期步骤。肺炎链球菌定植于鼻咽部并引起局部和全身性疾病。与从败血症或脑膜炎患者分离出的菌株相比，从中耳炎患者分离出的菌株具有更高的黏附能力，这表明与鼻咽部黏膜的结合在局部感染而非侵袭性感染中很重要。已对肺炎链球菌与上皮细胞结合的生化基础进行了研究。将人鼻咽上皮细胞与肺炎链球菌混合，并通过显微镜观察确定黏附情况。在添加上皮细胞之前，通过对肺炎链球菌进行预孵育来测试上皮细胞受体类似物对黏附的抑制作用。用代表上皮细胞表面受体结构的糖缀合物进行竞争性抑制，结果表明含有二糖GlcNAcbeta1连接到3Galbeta-的糖类具有受体功能。
