Evenson D P, Arlin Z, Welt S, Claps M L, Melamed M R
Cancer. 1984 Jan 1;53(1):30-6. doi: 10.1002/1097-0142(19840101)53:1<30::aid-cncr2820530108>3.0.co;2-w.
Six men with acute lymphocytic leukemia (ALL) were studied prospectively to assess the effect of treatment with the L-10 protocol on reproductive capacity. Before therapy three men had fathered children (two, two children; one, three children); the others were sexually mature although no fertility studies had been done prior to or during their treatment. Each patient had a minimum of 3 1/2 years of continuous chemotherapy as part of this study. Semen analysis was done 10 to 52 months (median, 31.5 months) after completion of therapy. One patient had lower than normal sperm concentration but near normal total sperm count and normal motility; the others had a normal sperm concentration and motility. Sperm nuclei were isolated from each sample and analyzed by flow cytometry for resistance to DNA denaturation in situ; all samples had relatively high resistance to denaturation, consistent with a normal, fertile reproductive status. After completion of therapy, one patient fathered a normal child, and a second patient fathered one child with multiple congenital malformations followed by a second child who was normal.
对6名急性淋巴细胞白血病（ALL）男性患者进行了前瞻性研究，以评估L-10方案治疗对生殖能力的影响。治疗前，3名男性已有子女（2名男性各有2个孩子；1名男性有3个孩子）；其他患者虽未在治疗前或治疗期间进行生育能力研究，但已性成熟。作为本研究的一部分，每位患者至少接受了3年半的持续化疗。在治疗结束后10至52个月（中位数为31.5个月）进行了精液分析。1名患者的精子浓度低于正常水平，但总精子数接近正常且活力正常；其他患者的精子浓度和活力正常。从每个样本中分离出精子细胞核，并通过流式细胞术分析其对原位DNA变性的抗性；所有样本对变性的抗性相对较高，这与正常的、可生育的生殖状态一致。治疗结束后，1名患者生育了一个正常孩子，另1名患者生育了一个患有多种先天性畸形的孩子，随后又生育了一个正常的孩子。
