人唾液中α-酮酸的高效液相色谱分析
High-performance liquid chromatography of alpha-keto acids in human saliva.
作者信息
Tsuchiya H, Hashizume I, Tokunaga T, Tatsumi M, Takagi N, Hayashi T
出版信息
Arch Oral Biol. 1983;28(11):989-92. doi: 10.1016/0003-9969(83)90052-3.
alpha-Keto acids in human mixed saliva collected without stimulation were analysed by reversed-phase ion-pair high-performance liquid chromatography (HPLC). Several alpha-keto acids were found in saliva and their concentrations were: alpha-ketoglutaric acid (KGA), 221 +/- 142; pyruvic acid (PA), 7490 +/- 5600; alpha-ketoisovaleric acid (KIVA), 61 +/- 23; alpha-ketoisocaproic acid (KICA), 137 +/- 79; alpha-keto-beta-methylvaleric acid (KMVA), 41 +/- 19 nmol/dl (mean +/- SD, n = 40). Their levels proved to be lower than those in plasma, except that of PA. Their concentrations in saliva showed individual variation compared with those in blood.
采用反相离子对高效液相色谱法（HPLC）分析了无刺激情况下采集的人混合唾液中的α-酮酸。在唾液中发现了几种α-酮酸，其浓度分别为：α-酮戊二酸（KGA），221±142；丙酮酸（PA），7490±5600；α-酮异戊酸（KIVA），61±23；α-酮异己酸（KICA），137±79；α-酮-β-甲基戊酸（KMVA），41±19 nmol/dl（平均值±标准差，n = 40）。除PA外，它们的水平均低于血浆中的水平。与血液中的浓度相比，它们在唾液中的浓度存在个体差异。
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