Bell R, Cotter S, Lillquist A, Sallan S, McCaffrey R
Blood. 1984 Feb;63(2):380-3.
The clinical significance of initial DEAE chromatography of glucocorticoid binders in lymphoblastic disease was evaluated in an animal model. Domestic cats and dogs with lymphoblastic disease were treated with prednisone, 2 mg/kg/day, for 14 days, and the outcome of therapy was correlated with DEAE chromatograms of glucocorticoid binders, using 3H-triamcinolone as ligand. Six of 30 animals had a single-peak low-salt binder species, similar to that seen in a subset of human leukemia, and none of these responded. Of the 29 animals with chromatograms identical to normal tissues, 6 had a complete response and another 11 a partial response. This distribution of responders is statistically significant (p = 0.02). Thus, the leukemia-associated single-peak DEAE species appears to be associated with glucocorticoid resistance, as defined by clinical responsiveness. In contrast, the two-peak normal pattern is a necessary, but insufficient, criterion for defining responsive disease.
在一个动物模型中评估了淋巴细胞性疾病中糖皮质激素结合物初始二乙氨基乙基（DEAE）色谱分析的临床意义。患有淋巴细胞性疾病的家猫和家犬接受泼尼松治疗，剂量为2毫克/千克/天，持续14天，使用³H-曲安西龙作为配体，将治疗结果与糖皮质激素结合物的DEAE色谱图相关联。30只动物中有6只具有单峰低盐结合物类型，类似于在一部分人类白血病中所见，这些动物均无反应。在29只色谱图与正常组织相同的动物中，6只完全缓解，另外11只部分缓解。这种反应者的分布具有统计学意义（p = 0.02）。因此，白血病相关的单峰DEAE类型似乎与糖皮质激素抵抗相关，如通过临床反应性所定义。相比之下，双峰正常模式是定义反应性疾病的必要但不充分标准。
