Kasukabe T, Honma Y, Hozumi M
J Cell Physiol. 1984 Feb;118(2):105-12. doi: 10.1002/jcp.1041180202.
The murine macrophage-like cell line (Mm-1), which is nonleukemogenic to syngeneic SL mice, was originally derived from spontaneously differentiated cells of a clonal line of mouse myeloid leukemia cells (M1). In the present experiment, variant cell lines with a high (Mm-A), moderate (Mm-P), and little or no (Mm-S1 and Mm-S2) leukemogenic potential were obtained from the Mm-1 cells. The mean survival times of syngeneic SL mice inoculated i.p. with 5 X 10(6) Mm-A and Mm-P cells were 17 and 33 days, respectively, whereas almost all the mice inoculated with Mm-S1 or Mm-S2 cells survived for more than 90 days. These variant cell lines did not lose their macrophage-like characteristics in vitro. These variant cell lines phagocytized latex beads and sensitized sheep erythrocytes, produced lysozyme, and adhered to culture dishes. The four variant cell lines showed no significant difference in proliferation rates in vitro in liquid medium containing 10% calf serum, but Mm-A cells could grow both in soft agar medium in the absence of ascitic fluid containing colony-stimulating factor (CSF) and in liquid medium containing 1% serum, whereas Mm-P cells could grow in the liquid medium but not in soft agar medium without ascitic fluid, and Mm-S1 and Mm-S2 cells could not grow in either medium. The ratio of the nuclear area to the cell area (NCR) of Mm-A cells was a high (51%) but those of Mm-S1 and Mm-S2 cells were low (40-41%), and that of Mm-P cells was intermediate (44%). The leukemogenicity of Mm-1 cell lines was roughly correlated with their NCR. The possibility that interactions between Mm-1 variant cells and host immune cells might be involved in the mechanisms of their different leukemogenicities was not supported by results on the in vitro susceptibilities of Mm-1 variant cells to the cytostatic actions by normal macrophages and spleen cells and on leukemogenicities of the Mm-1 variant cells in athymic nude mice. A possible method of control of the leukemogenicity of Mm-1 variant cells is discussed.
对同基因SL小鼠无致白血病性的鼠巨噬细胞样细胞系（Mm-1），最初源自小鼠髓系白血病细胞（M1）克隆系的自发分化细胞。在本实验中，从Mm-1细胞获得了具有高致白血病潜能（Mm-A）、中度致白血病潜能（Mm-P）以及低或无致白血病潜能（Mm-S1和Mm-S2）的变异细胞系。经腹腔接种5×10⁶个Mm-A和Mm-P细胞的同基因SL小鼠的平均存活时间分别为17天和33天，而接种Mm-S1或Mm-S2细胞的几乎所有小鼠存活超过90天。这些变异细胞系在体外并未丧失其巨噬细胞样特征。这些变异细胞系吞噬乳胶珠和致敏绵羊红细胞，产生溶菌酶，并黏附于培养皿。这四种变异细胞系在含10%小牛血清的液体培养基中的体外增殖率无显著差异，但Mm-A细胞既能在不含集落刺激因子（CSF）腹水的软琼脂培养基中生长，也能在含1%血清的液体培养基中生长，而Mm-P细胞能在液体培养基中生长，但在无腹水的软琼脂培养基中不能生长，Mm-S1和Mm-S2细胞在这两种培养基中均不能生长。Mm-A细胞的核面积与细胞面积之比（NCR）较高（51%），而Mm-S1和Mm-S2细胞的该比值较低（40 - 41%），Mm-P细胞的该比值处于中间水平（44%）。Mm-1细胞系的致白血病性与其NCR大致相关。Mm-1变异细胞与宿主免疫细胞之间的相互作用可能参与其不同致白血病机制的可能性，未得到Mm-1变异细胞对正常巨噬细胞和脾细胞的细胞抑制作用的体外敏感性结果以及Mm-1变异细胞在无胸腺裸鼠中的致白血病性结果的支持。本文讨论了一种控制Mm-1变异细胞致白血病性的可能方法。
