Balinsky D, Platz C E, Lewis J W
J Natl Cancer Inst. 1984 Feb;72(2):217-24.
The activities of six different enzymes were compared in 29 normal, 34 dysplastic, and 80 cancerous (both primary and metastatic) human breast tissues; in MCF-7 cells; and in primary rat mammary tumors. Benign lesions generally showed enzyme activities similar to those of normal breast tissues. Malignant tumors had significantly increased activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), fructose-bisphosphate aldolase, hexokinase (HK), pyruvate kinase (PK), and creatine kinase. Enzyme activity in the malignant tumor was always higher than that in apparently normal or fibrocystic tissue from the same patient. Enzyme activities did not correlate with the levels of estrogen and progesterone receptors. LDH, MDH, and HK were elevated to a similar extent in all the tissues examined. Conversely, PK was elevated to a much greater extent in cancerous tissues, particularly in MCF-7 cells. The elevated activities of these enzymes may have diagnostic potential, especially when tumor tissue and apparently normal tissue from the same patient are compared.
在29份正常、34份发育异常以及80份癌性（原发性和转移性）人类乳腺组织中，对六种不同酶的活性进行了比较；同时也在MCF-7细胞以及原发性大鼠乳腺肿瘤中进行了比较。良性病变通常显示出与正常乳腺组织相似的酶活性。恶性肿瘤的乳酸脱氢酶（LDH）、苹果酸脱氢酶（MDH）、果糖二磷酸醛缩酶、己糖激酶（HK）、丙酮酸激酶（PK）和肌酸激酶的活性显著增加。恶性肿瘤中的酶活性总是高于同一患者明显正常或纤维囊性组织中的酶活性。酶活性与雌激素和孕激素受体水平无关。在所检查的所有组织中，LDH、MDH和HK的升高程度相似。相反，PK在癌性组织中，尤其是在MCF-7细胞中升高程度更大。这些酶活性的升高可能具有诊断潜力，特别是当比较同一患者的肿瘤组织和明显正常组织时。
