Ber R, Lanir N
J Natl Cancer Inst. 1984 Feb;72(2):403-9.
The double-mutant cell line 4T00.1 is derived from a plasmacytoma of a BALB/c mouse and is resistant to 6-thioguanine and ouabain. These cells were inoculated into (BALB/c X C57BL)F1 mice by different routes--sc, ip, intrasplenically, and intrathymically. The degree of tumorigenicity and pattern of tumor development were site-dependent. Intrasplenic inoculation of 10(3)-10(4) 4T00.1 cells resulted in development of large omental tumors accompanied by marked ascites. Tenfold to a thousandfold more 4T00.1 cells were required to obtain tumors by other routes. From all solid tumors and ascites and from various organs of tumor-bearing mice, which were explanted into culture in double selective medium containing hypoxanthine, aminopterin, and thymidine plus ouabain (in which only hybrids between 4T00.1 and normal cells can survive), proliferating and nonproliferating cultures were obtained. Of the 14 proliferating cultures, 9 proved to be hybrids by chromosome and H-2 isoantigen analyses and were tumorigenic when 10(6) cells were inoculated sc into syngeneic F1 mice.
双突变细胞系4T00.1源自一只BALB/c小鼠的浆细胞瘤，对6-硫鸟嘌呤和哇巴因具有抗性。这些细胞通过不同途径接种到（BALB/c×C57BL）F1小鼠体内——皮下、腹腔内、脾内和胸腺内。致瘤性程度和肿瘤发展模式取决于接种部位。脾内接种10³ - 10⁴个4T00.1细胞会导致大网膜肿瘤形成并伴有明显腹水。通过其他途径获得肿瘤则需要多10倍至1000倍的4T00.1细胞。从所有实体瘤、腹水以及荷瘤小鼠的各个器官中取出组织，接种到含有次黄嘌呤、氨基蝶呤、胸腺嘧啶核苷和哇巴因的双重选择培养基（只有4T00.1细胞与正常细胞的杂交体能够存活）中进行培养，获得了增殖和非增殖培养物。在14个增殖培养物中，通过染色体和H-2同种抗原分析，有9个被证明是杂交体，当将10⁶个细胞皮下接种到同基因F1小鼠体内时具有致瘤性。
