关于乙酰胆碱受体的溶剂萃取
On the solvent-extraction of acetylcholine receptor.
作者信息
Christopher J P, Millar D B, Grafius M A
出版信息
Experientia. 1978 Jun 15;34(6):689-91. doi: 10.1007/BF01947259.
PMID:658271
Abstract
Chloroform/methanol rechromatography on Sephadex LH-20 of fractions isolated from organic solvent extracts of E. electricus electric organs exhibited alterations in elution point compared to the original chromatography. Additionally, 3H decamethonium bromide did not coelute with any of the observed fractions. It is concluded that as presently described the organic solvent isolation procedure for the cholinergic receptor exhibits undesirable characteristics.
摘要
对电鳗（E. electricus）电器官的有机溶剂提取物分离得到的各组分进行氯仿/甲醇在Sephadex LH - 20上的再色谱分析，结果显示与原始色谱相比，洗脱点发生了变化。此外，3H溴化十烃季铵与任何观察到的组分都没有共同洗脱。结论是，就目前所描述的而言，胆碱能受体的有机溶剂分离程序具有不良特性。
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