Ads A H, Piddington R, Goldman A S, Herold R
Arch Oral Biol. 1983;28(12):1115-9. doi: 10.1016/0003-9969(83)90167-x.
The lysosomal enzymes, non-specific esterases, beta-glucuronidase, N-acetyl-beta-glucosaminidase and aryl sulphatases A and B, were examined histochemically in medial-edge epithelia (MEE) of single palatal shelves in vitro. Activities of most enzymes increased gradually in MEE with a peak at 24-26 h of culture. Aryl sulphatase B activities were lower than the others and aryl sulphatase A activities could not be detected in the palatal cells during the entire culture period. By 48 h, MEE cells degenerated and were lost. Cortisol suppressed increased activities of these hydrolytic enzymes and prevented programmed breakdown of the palatal epithelium.
对体外培养的单个腭突内侧边缘上皮（MEE）中的溶酶体酶、非特异性酯酶、β-葡萄糖醛酸酶、N-乙酰-β-氨基葡萄糖苷酶以及芳基硫酸酯酶A和B进行了组织化学检测。大多数酶的活性在MEE中逐渐升高，在培养24 - 26小时时达到峰值。芳基硫酸酯酶B的活性低于其他酶，并且在整个培养期间腭细胞中均未检测到芳基硫酸酯酶A的活性。到48小时时，MEE细胞发生退化并消失。皮质醇抑制了这些水解酶活性的增加，并阻止了腭上皮的程序性分解。
