Thomas H F, Carella P
Arch Oral Biol. 1983;28(12):1125-30. doi: 10.1016/0003-9969(83)90169-3.
Twelve fully-formed human third molars were obtained immediately after extraction, the roots removed, the crowns split into buccal and lingual portions fixed in Karnovsky solution and either left in the mineralized state or demineralized in a formic/citric acid solution. Specimens were dehydrated, critical point dried and examined by SEM. Demineralization of the teeth resulted in the loss of peritubular dentine and the appearance within each tubule of a sheet-like structure which extended from the predentine-dentine junction to the dentine-enamel junction. This sheet-like structure, here termed the lamina limitans, may play a role in the control of peritubular dentine formation.
十二颗完整的人类第三磨牙在拔除后立即获取，去除牙根，将牙冠劈成颊侧和舌侧部分，固定于卡诺夫斯基溶液中，要么保持矿化状态，要么在甲酸/柠檬酸溶液中脱矿。标本经脱水、临界点干燥后用扫描电子显微镜检查。牙齿脱矿导致管周牙本质丧失，并且在每个牙小管内出现从前期牙本质 - 牙本质交界处延伸至牙本质 - 釉质交界处的片状结构。这种片状结构，在此称为界限板，可能在管周牙本质形成的控制中起作用。
