Nagata K, Sagara J, Ichikawa Y
Cell Struct Funct. 1983 Jun;8(2):171-83. doi: 10.1247/csf.8.171.
Gelation of extracts of a myeloid leukemia cell line (Ml) was compared before and after differentiation induced with conditioned medium (CM) from rat embryo cells. Although an extract of Mml cells, a macrophage line derived from Ml line, gelled when warmed in the presence of 2 mM MgCl2, undifferentiated Ml cells gelled only after dialysis and a supplement of exogenous actin. After differentiation had been induced, an addition of exogenous actin, but not dialysis, was needed for gelation. Small amounts of KCl always inhibited the gelation of the control Ml cell extracts, but they promoted gelation of the CM-treated Ml and Mml cell extracts. Thus, the dialysis required for gelation of the control Ml cell extract appears to be necessary for the exclusion of endogenous KCl. Several possible mechanisms for the KCl control of gelation, as well as different requirements of exogenous actin needed for gelation are discussed based on the results of our experiments.
对髓系白血病细胞系（Ml）提取物在大鼠胚胎细胞条件培养基（CM）诱导分化前后的凝胶化情况进行了比较。尽管源自Ml系的巨噬细胞系Mml细胞的提取物在2 mM MgCl₂存在下加热时会凝胶化，但未分化的Ml细胞仅在透析并补充外源性肌动蛋白后才会凝胶化。诱导分化后，凝胶化需要添加外源性肌动蛋白，而不是透析。少量的KCl总是会抑制对照Ml细胞提取物的凝胶化，但它们会促进经CM处理的Ml和Mml细胞提取物的凝胶化。因此，对照Ml细胞提取物凝胶化所需的透析似乎是排除内源性KCl所必需的。基于我们的实验结果，讨论了KCl控制凝胶化的几种可能机制以及凝胶化所需外源性肌动蛋白的不同要求。
