一种使用固定化酶检测血清中总3α-羟基胆汁酸的生物发光测定法。

A bioluminescence assay for total 3 alpha-hydroxy bile acids in serum using immobilized enzymes.

作者信息

Schoelmerich J, van Berge Henegouwen G P, Hofmann A F, DeLuca M

出版信息

Clin Chim Acta. 1984 Feb 14;137(1):21-32. doi: 10.1016/0009-8981(84)90308-5.

Abstract

A bioluminescence assay for bile acids was developed using a co-immobilized 3 alpha-hydroxysteroid dehydrogenase, diaphorase, and bacterial luciferase. The assay was specific for bile acids containing a free 3 alpha-hydroxyl group, as well as androsterone. Light output was linear over a bile acid concentration range of 1-20 000 pmol. Intra-assay precision was 6.2-8.2% and the recovery of added standards was 92-110%. Comparison of results using the bioluminescence assay with those using gas liquid chromatography revealed an excellent correlation (r = 0.99, n = 31). Since the bioluminescence assay is rapid, sensitive, specific, and uses inexpensive reagents, it appears to be an ideal method for the measurement of total bile acids in serum.

摘要

利用共固定化的3α-羟基类固醇脱氢酶、黄递酶和细菌荧光素酶开发了一种胆汁酸生物发光测定法。该测定法对含有游离3α-羟基的胆汁酸以及雄甾酮具有特异性。在1 - 20000 pmol的胆汁酸浓度范围内，光输出呈线性。批内精密度为6.2 - 8.2%，添加标准品的回收率为92 - 110%。将生物发光测定法的结果与气相色谱法的结果进行比较，发现具有极好的相关性（r = 0.99，n = 31）。由于生物发光测定法快速、灵敏、特异且使用廉价试剂，它似乎是测定血清中总胆汁酸的理想方法。