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[通过激光散射比浊法估算细胞凝集]

[Estimation of cell agglutination by laser nephelometry].

作者信息

Viadro M M, Osipov S G

出版信息

Biull Eksp Biol Med. 1984 Mar;97(3):360-2.

PMID:6584183
Abstract

Laser nephelometry was used to measure the lectin-mediated agglutination of cultured human tumor cells (of osteosarcoma and adenocarcinoma of the lungs). The rates of a decrease in the value of light dispersion (V delta VLD) and the length of time (to) necessary for complete cell sedimentation (at V delta VLD-O) can be used as quantitative indices of cell agglutination. V delta VLD and to were found to correlate with the concentrations of tumor cells and lectins (PHA and Con A).

摘要

采用激光散射比浊法测定凝集素介导的培养人肿瘤细胞(骨肉瘤和肺腺癌)的凝集反应。光散射值降低率(VΔVLD)以及细胞完全沉降所需的时间(to)(VΔVLD为0时)可作为细胞凝集的定量指标。发现VΔVLD和to与肿瘤细胞及凝集素(PHA和Con A)的浓度相关。

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