甘油处理的骨骼肌收缩中横桥排列的证据。
Evidence for cross-bridge order in contraction of glycerinated skeletal muscle.
作者信息
Burghardt T P, Ando T, Borejdo J
出版信息
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7515-9. doi: 10.1073/pnas.80.24.7515.
Abstract
The linear dichroism of iodoacetylrhodamine labels attached to the single reactive thiol groups of myosin heads was measured to determine the spatial orientation of myosin cross-bridges in single glycerinated skeletal muscle fibers. We have shown previously that in rigor the chromophoric labels are well ordered and assume an orientation nearly perpendicular to the fiber axis; in the presence of MgADP, a large fraction of probe remains well ordered but the probe attitude assumes a more slanted orientation; in relaxed muscle, the probe order is largely lost, implying a high degree of cross-bridge disorder. In this paper, we report that during isometric contraction a large fraction of the probe shows a high degree of order, suggesting the attachment of approximately equal to 65% of the cross-bridges to actin. These ordered cross-bridges have a probe attitude similar to that of the MgADP-induced static state and hence are in a mechanical state quite distinct from rigor.
摘要
通过测量附着在肌球蛋白头部单个反应性巯基上的碘乙酰罗丹明标签的线性二色性，来确定单根甘油化骨骼肌纤维中肌球蛋白横桥的空间取向。我们之前已经表明，在僵直状态下，发色团标签排列良好，其取向几乎垂直于纤维轴；在MgADP存在的情况下，大部分探针仍排列良好，但探针的姿态呈现出更倾斜的取向；在松弛的肌肉中，探针的有序性基本丧失，这意味着横桥高度无序。在本文中，我们报告在等长收缩过程中，大部分探针显示出高度的有序性，这表明约65%的横桥附着于肌动蛋白。这些有序的横桥具有与MgADP诱导的静态相似的探针姿态，因此处于与僵直状态截然不同的机械状态。
相似文献
1
Evidence for cross-bridge order in contraction of glycerinated skeletal muscle.甘油处理的骨骼肌收缩中横桥排列的证据。
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7515-9. doi: 10.1073/pnas.80.24.7515.
2
Observation of two orientations from rigor cross-bridges in glycerinated muscle fibers.甘油化肌纤维中僵直横桥两种取向的观察。
Biochemistry. 1986 Oct 7;25(20):6203-7. doi: 10.1021/bi00368a055.
3
Crossbridge order and orientation in resting single glycerinated muscle fibres studied by linear dichroism of bound rhodamine labels.通过结合罗丹明标记物的线性二色性研究静息状态下单根甘油处理肌纤维中的横桥排列与取向
J Muscle Res Cell Motil. 1984 Dec;5(6):657-63. doi: 10.1007/BF00713924.
4
Fraction of myosin cross-bridges bound to actin in active muscle fibers: estimation by fluorescence anisotropy measurements.活性肌纤维中与肌动蛋白结合的肌球蛋白横桥比例：通过荧光各向异性测量进行估算
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8478-82. doi: 10.1073/pnas.82.24.8478.
5
Orientation of spin-labeled light chain-2 exchanged onto myosin cross-bridges in glycerinated muscle fibers.自旋标记的轻链-2与甘油处理的肌纤维中肌球蛋白横桥的交换方向。
Biophys J. 1991 Jan;59(1):127-38. doi: 10.1016/S0006-3495(91)82205-6.
6
Muscle cross-bridges: do they rotate?肌肉横桥：它们会旋转吗？
Adv Exp Med Biol. 1984;170:413-27. doi: 10.1007/978-1-4684-4703-3_37.
7
Cross-bridge scheme and force per cross-bridge state in skinned rabbit psoas muscle fibers.去皮肤兔腰大肌纤维中的横桥模式及每个横桥状态下的力
Biophys J. 1993 Aug;65(2):638-51. doi: 10.1016/S0006-3495(93)81109-3.
8
Path and extent of cross-bridge rotation during muscle contraction.肌肉收缩过程中横桥旋转的路径和范围。
Biochemistry. 1994 May 10;33(18):5382-91. doi: 10.1021/bi00184a005.
9
The effects of pyrophosphate on myosin heads and on fiber stiffness.焦磷酸盐对肌球蛋白头部及纤维硬度的影响。
Adv Exp Med Biol. 1988;226:255-65.
10
Probe studies of the MgADP state of muscle cross-bridges: microscopic and wavelength-dependent fluorescence polarization from 1,5-IAEDANS-labeled myosin subfragment 1 decorating muscle fibers.肌肉横桥MgADP状态的探针研究：来自标记于肌纤维上的1,5-IAEDANS标记肌球蛋白亚片段1的微观及波长依赖性荧光偏振
Biochemistry. 1987 Jul 14;26(14):4517-23. doi: 10.1021/bi00388a052.
引用本文的文献
1
Studies on the impellers generating force in muscle.关于肌肉中产生力量的叶轮的研究。
Biophys Rev. 2020 Aug;12(4):767-769. doi: 10.1007/s12551-020-00705-5. Epub 2020 Jun 11.
2
Effect of a myosin regulatory light chain mutation K104E on actin-myosin interactions.肌球蛋白调节轻链突变K104E对肌动蛋白-肌球蛋白相互作用的影响。
Am J Physiol Heart Circ Physiol. 2015 May 15;308(10):H1248-57. doi: 10.1152/ajpheart.00834.2014. Epub 2015 Mar 13.
3
Myosin head rotation in muscle fibers measured using polarized fluorescence photobleaching recovery.使用偏振荧光光漂白恢复测量肌纤维中的肌球蛋白头部旋转。
J Fluoresc. 1995 Dec;5(4):355-67. doi: 10.1007/BF01152562.
4
Evidence for pre- and post-power stroke of cross-bridges of contracting skeletal myofibrils.收缩肌原纤维的交联桥的预功和后功的证据。
Biophys J. 2011 Feb 16;100(4):1024-33. doi: 10.1016/j.bpj.2011.01.007.
5
GFP-tagged regulatory light chain monitors single myosin lever-arm orientation in a muscle fiber.绿色荧光蛋白标记的调节轻链监测肌肉纤维中单个肌球蛋白杠杆臂的方向。
Biophys J. 2007 Sep 15;93(6):2226-39. doi: 10.1529/biophysj.107.107433. Epub 2007 May 18.
6
In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy.利用金属膜增强全内反射显微镜进行原位荧光蛋白成像。
Biophys J. 2006 Jun 15;90(12):4662-71. doi: 10.1529/biophysj.105.079442. Epub 2006 Mar 24.
7
Steady-state fluorescence polarization studies of the orientation of myosin regulatory light chains in single skeletal muscle fibers using pure isomers of iodoacetamidotetramethylrhodamine.使用碘乙酰胺基四甲基罗丹明的纯异构体对单根骨骼肌纤维中肌球蛋白调节轻链的取向进行稳态荧光偏振研究。
Biophys J. 1998 Jun;74(6):3083-92. doi: 10.1016/S0006-3495(98)78015-4.
8
Luminescence resonance energy transfer measurements in myosin.肌球蛋白中的发光共振能量转移测量
Biophys J. 1998 May;74(5):2451-8. doi: 10.1016/s0006-3495(98)77953-6.
9
Fluorescence polarization of skeletal muscle fibers labeled with rhodamine isomers on the myosin heavy chain.用若丹明异构体标记肌球蛋白重链的骨骼肌纤维的荧光偏振。
Biophys J. 1996 Dec;71(6):3330-43. doi: 10.1016/S0006-3495(96)79526-7.
10
Orientation changes in myosin regulatory light chains following photorelease of ATP in skinned muscle fibers.在去皮肤肌纤维中ATP光释放后肌球蛋白调节轻链的取向变化
Biophys J. 1996 Apr;70(4):1847-62. doi: 10.1016/S0006-3495(96)79750-3.
本文引用的文献
1
Relaxation of muscle fibres by photolysis of caged ATP.通过光解笼化ATP使肌纤维松弛。
Nature. 1982 Dec 23;300(5894):701-5. doi: 10.1038/300701a0.
2
Cross-bridge orientation in skeletal muscle measured by linear dichroism of an extrinsic chromophore.通过外在发色团的线性二色性测量骨骼肌中的横桥取向。
J Mol Biol. 1982 Jul 5;158(3):391-414. doi: 10.1016/0022-2836(82)90205-4.
3
Millisecond time-resolved changes in x-ray reflections from contracting muscle during rapid mechanical transients, recorded using synchrotron radiation.利用同步辐射记录的在快速机械瞬变期间收缩肌肉的X射线反射的毫秒级时间分辨变化。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2297-301. doi: 10.1073/pnas.78.4.2297.
4
Orientation of spin labels attached to cross-bridges in contracting muscle fibres.收缩肌纤维中附着于横桥的自旋标记物的取向。
Nature. 1982 Dec 23;300(5894):776-8. doi: 10.1038/300776a0.
5
Orientation of spin-labeled myosin heads in glycerinated muscle fibers.甘油化肌纤维中自旋标记肌球蛋白头部的取向
Biophys J. 1980 Dec;32(3):891-906. doi: 10.1016/S0006-3495(80)85024-7.
6
Submillisecond rotational dynamics of spin-labeled myosin heads in myofibrils.肌原纤维中自旋标记肌球蛋白头部的亚毫秒级旋转动力学。
Biophys J. 1980 Dec;32(3):873-89. doi: 10.1016/S0006-3495(80)85023-5.
7
The low-angle x-ray diagram of vertebrate striated muscle and its behaviour during contraction and rigor.脊椎动物横纹肌的低角度X射线图及其在收缩和强直过程中的表现。
J Mol Biol. 1967 Dec 14;30(2):383-434. doi: 10.1016/s0022-2836(67)80046-9.
8
Polarization of tryptophan fluorescence in muscle.肌肉中色氨酸荧光的偏振
Biochemistry. 1969 Nov;8(11):4517-22. doi: 10.1021/bi00839a044.
9
Letter: Crossbridge angle when ADP is bound to myosin.信函：当二磷酸腺苷与肌球蛋白结合时的横桥角度。
J Mol Biol. 1974 Jun 25;86(2):495-7. doi: 10.1016/0022-2836(74)90033-3.
10
Polarization of fluorescence from single skinned glycerinated rabbit psoas fibers in rigor and relaxation.处于强直收缩和舒张状态的单个剥除肌膜的甘油处理兔腰大肌纤维的荧光偏振
Biochim Biophys Acta. 1977 Mar 11;459(3):578-95. doi: 10.1016/0005-2728(77)90056-1.
Zotero 插件