中国仓鼠卵巢细胞中的染色体重排与基因表达：中国仓鼠卵巢细胞Z3、Z4、Z5和Z7号染色体上八个酶基因座等位基因的定位

Chromosomal rearrangements and gene expression in CHO cells: mapping of alleles for eight enzyme loci on CHO chromosomes Z3, Z4, Z5, and Z7.

作者信息

Adair G M, Stallings R L, Siciliano M J

出版信息

Somat Cell Mol Genet. 1984 May;10(3):283-95. doi: 10.1007/BF01535250.

Abstract

Analysis of CHO electrophoretic mobility shift mutants for six enzyme loci ( LDHA , GAA, IDH2 , ME1, PGM3, and MPI) that have been previously mapped to Chinese hamsters chromosomes 3 and 4 indicated that each of these loci, with the exception of IDH2 , are functionally dizygous in CHO. Segregation analysis of CHO X mouse somatic cell hybrids allowed regional gene mapping assignments for a total of eight Chinese hamster chromosome 3- or 4-derived marker loci (the above six, plus APRT and PKM2) to CHO chromosomes Z3 , Z4 , Z5 , and Z7 . For seven of these enzyme loci (all but IDH2 ), two alleles are expressed in CHO cells, each segregating with a different Z-group chromosome. These gene mapping assignments confirm genetically that CHO chromosomes Z3 , Z4 , Z5 , and Z7 are, in fact, derived from Chinese hamster chromosomes 3 and 4, and provide insight into the effects of chromosomal rearrangements on gene expression and hemizygosity in CHO cells.

摘要

对先前已定位到中国仓鼠3号和4号染色体上的六个酶基因座（LDHA、GAA、IDH2、ME1、PGM3和MPI）的CHO电泳迁移率变动突变体进行分析，结果表明，除IDH2外，这些基因座在CHO中功能上都是杂合的。对CHO与小鼠体细胞杂种的分离分析，使得总共八个源自中国仓鼠3号或4号染色体的标记基因座（上述六个，加上APRT和PKM2）能够被定位到CHO染色体Z3、Z4、Z5和Z7上。对于其中七个酶基因座（除IDH2外的所有基因座），两个等位基因在CHO细胞中表达，每个等位基因与不同的Z组染色体分离。这些基因定位结果从遗传学上证实，CHO染色体Z3、Z4、Z5和Z7实际上源自中国仓鼠染色体3和4，并为染色体重排对CHO细胞中基因表达和半合子状态的影响提供了见解。