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中国仓鼠卵巢细胞染色体重排的遗传效应：TK、GALK、ACP1、ADA和ITPA基因座的表达及染色体定位

Genetic effects of chromosomal rearrangements in Chinese hamster ovary cells: expression and chromosomal assignment of TK, GALK, ACP1, ADA, and ITPA loci.

作者信息

Stallings R L, Adair G M, Siciliano J, Greenspan J, Siciliano M J

出版信息

Mol Cell Biol. 1983 Nov;3(11):1967-74. doi: 10.1128/mcb.3.11.1967-1974.1983.

DOI:10.1128/mcb.3.11.1967-1974.1983
PMID:6656761
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC370064/
Abstract

Polyethylene glycol-mediated fusion of Chinese hamster ovary (CHO) cells with mouse Cl1D cells produced interspecific somatic cell hybrids which slowly segregated CHO chromosomes. Cytogenetic and isozyme analysis of HAT- and bromodeoxyuridine-selected hybrid subclones and of members of a hybrid clone panel retaining different combinations of CHO chromosomes enabled provisional assignments of the following enzyme loci to CHO chromosomes: TK, GALK, and ACP1 to chromosome 7; TK and GALK to chromosome Z13; ACP1, ADA, and ITPA to chromosome Z8; and ADA and ITPA to chromosome Z9. These genetic markers reflect the origin of each of these Z group chromosomes and indicate the functional activity of alleles located on rearranged chromosomes. Identification of diploid electrophoretic shift mutations for ADA and ITPA was consistent with those observations. Assignment of the functional TK locus in TK+/- CHO-AT3-2 cells indicated that gene deletion may be responsible for TK hemizygosity in this subline.

摘要

聚乙二醇介导的中国仓鼠卵巢（CHO）细胞与小鼠Cl1D细胞融合产生了种间体细胞杂种，这些杂种会缓慢分离CHO染色体。对经次黄嘌呤-氨基蝶呤-胸腺嘧啶核苷（HAT）和溴脱氧尿苷筛选的杂交亚克隆以及保留不同CHO染色体组合的杂交克隆组中的成员进行细胞遗传学和同工酶分析，使得能够将以下酶基因座初步定位到CHO染色体上：胸苷激酶（TK）、半乳糖激酶（GALK）和酸性磷酸酶1（ACP1）定位于染色体7；TK和GALK定位于染色体Z13；ACP1、腺苷脱氨酶（ADA）和次黄嘌呤-鸟嘌呤磷酸核糖转移酶（ITPA）定位于染色体Z8；ADA和ITPA定位于染色体Z9。这些遗传标记反映了这些Z组染色体中每一条的起源，并表明了位于重排染色体上的等位基因的功能活性。对ADA和ITPA的二倍体电泳迁移突变的鉴定与这些观察结果一致。在TK+/- CHO-AT3-2细胞中功能性TK基因座的定位表明，基因缺失可能是该亚系中TK半合子状态的原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/9aa6194585e4/molcellb00111-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/5a3668bdb0d4/molcellb00111-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/c76a92ced8d7/molcellb00111-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/9aa6194585e4/molcellb00111-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/5a3668bdb0d4/molcellb00111-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/c76a92ced8d7/molcellb00111-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edf8/370064/9aa6194585e4/molcellb00111-0091-b.jpg

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Genetic effects of chromosomal rearrangements in Chinese hamster ovary cells: expression and chromosomal assignment of TK, GALK, ACP1, ADA, and ITPA loci.中国仓鼠卵巢细胞染色体重排的遗传效应：TK、GALK、ACP1、ADA和ITPA基因座的表达及染色体定位
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引用本文的文献

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本文引用的文献

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THE USE OF GENETIC VARIATION IN THE ANALYSIS OF ISOZYME STRUCTURE.遗传变异在同工酶结构分析中的应用。
Brookhaven Symp Biol. 1964 Dec;17:117-30.
2
EFFECT OF HALOGENATED PYRIMIDINES AND THYMIDINE ON GROWTH OF L-CELLS AND A SUBLINE LACKING THYMIDINE KINASE.卤代嘧啶和胸腺嘧啶对L细胞及缺乏胸苷激酶的亚系细胞生长的影响
Exp Cell Res. 1964 Jan;33:19-28. doi: 10.1016/s0014-4827(64)81006-5.
3
Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 7.将15个酶基因座确定、临时定位和/或区域定位到中国仓鼠1号、2号和7号常染色体上。
Somatic Cell Genet. 1981 Nov;7(6):683-98. doi: 10.1007/BF01538757.
4
Genetic polymorphisms and gene expression variations at enzyme loci in chinese hamster cell lines.中国仓鼠细胞系中酶基因座的遗传多态性和基因表达变异
Somatic Cell Genet. 1981 May;7(3):295-306. doi: 10.1007/BF01538855.
5
Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes.在中国仓鼠培养细胞中，参与蛋白质合成的两个基因emtB和leuS的连锁以及具有三个连锁基因突变的细胞系的分离。
J Cell Biol. 1980 Dec;87(3 Pt 1):697-702. doi: 10.1083/jcb.87.3.697.
6
Assignment of ADA, ITPA, AK1, and AK2 to Chinese hamster chromosomes. Genetic and structural evidence for the conservation of mammalian autosomal synteny.将ADA、ITPA、AK1和AK2定位到中国仓鼠染色体上。哺乳动物常染色体同线性保守性的遗传学和结构证据。
J Hered. 1982 Nov-Dec;73(6):399-404. doi: 10.1093/oxfordjournals.jhered.a109687.
7
Structural and functional hemi- and dizygous Chinese hamster chromosome 2 gene loci in CHO cells.中国仓鼠2号染色体基因位点在CHO细胞中的结构和功能半合子及二合子情况。
Somatic Cell Genet. 1982 Jul;8(4):413-22. doi: 10.1007/BF01538704.
8
Linkage of genetic markers emt and chr in Chinese hamster cells.中国仓鼠细胞中遗传标记emt和chr的连锁
Somatic Cell Genet. 1980 Mar;6(2):215-224. doi: 10.1007/BF01538797.
9
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10
Electrophoretically detectable mutations induced in CHO cells by varying doses of ultraviolet radiation.不同剂量紫外线辐射诱导中国仓鼠卵巢细胞产生的可通过电泳检测到的突变
Mutat Res. 1983 Jan;107(1):167-76. doi: 10.1016/0027-5107(83)90086-6.