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通过冷冻断裂揭示的人牙釉质器官细胞中的细胞间连接。

Intercellular junctions in the cells of the human enamel organ as revealed by freeze-fracture.

作者信息

Sasaki T, Segawa K, Takiguchi R, Higashi S

出版信息

Arch Oral Biol. 1984;29(4):275-86. doi: 10.1016/0003-9969(84)90101-8.

DOI:10.1016/0003-9969(84)90101-8
PMID:6586124
Abstract

Examined by thin sections and freeze-fracture replication techniques, secretory ameloblasts possessed two sets of the junctional complexes at both proximal and distal ends of the cell bodies, which consisted of tight junctions and occasional gap junctions and desmosomes. The proximal tight junction was fascia occludens, whereas the distal tight junction was zonula occludens. Between adjacent ameloblasts, mature gap junctions were frequent. The stratum-intermedium cells were connected to each other and to the stellate-reticulum cells and ameloblasts by well-developed desmosomes, gap junctions and fascia or macula-type tight junctions. Stellate-reticulum cells were inter-connected by many extensive cytoplasmic processes, in which well-developed desmosomes, small gap junctions and occasional macula-type tight junctions appeared. Thus fascia or macula-type tight junctions as well as many desmosomes seem to serve in mechanical, cell-to-cell adhesion during tooth formation. Frequent and large gap junctions between adjacent stratum-intermedium cells and between the stratum intermedium and the base of the ameloblast suggest that, in relation to enamel formation, these two cell layers form a functional unit.

摘要

通过薄切片和冷冻断裂复型技术检查发现,分泌性成釉细胞在细胞体的近端和远端均具有两组连接复合体,其由紧密连接以及偶尔出现的缝隙连接和桥粒组成。近端紧密连接为闭锁小带,而远端紧密连接为紧密连接带。相邻成釉细胞之间成熟的缝隙连接很常见。中间层细胞通过发育良好的桥粒、缝隙连接以及闭锁小带或斑状紧密连接相互连接,并与星网状细胞和成釉细胞相连。星网状细胞通过许多广泛的细胞质突起相互连接,其中出现了发育良好的桥粒、小缝隙连接以及偶尔的斑状紧密连接。因此,闭锁小带或斑状紧密连接以及许多桥粒似乎在牙齿形成过程中起到机械性细胞间黏附的作用。相邻中间层细胞之间以及中间层与成釉细胞基部之间频繁且大的缝隙连接表明,就釉质形成而言,这两个细胞层形成了一个功能单位。

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