Schlegel W, Krüger S, Korte K
FEBS Lett. 1984 Jun 4;171(1):141-4. doi: 10.1016/0014-5793(84)80475-5.
Prostaglandin E2-9- oxoreductase (PGE2-9-OR), the enzyme which converts prostaglandin E2 (PGE2) to prostaglandin F2 alpha (PGF2 alpha), has been detected in human decidua vera. A 105-fold purification was achieved when the centrifuged homogenate was fractionated sequentially by DEAE-Trisacryl, hydroxyapatite-agarose gel, ultrogel AcA 44 and Matrex gel blue A gel chromatographies. The following kinetic constants for PGE2-9-OR have been obtained. The equilibrium constant with respect to PGE2 is 83 microM, the Michaelis constant, Km, for PGE2 is 80 microM, for NADPH 1.6 microM. The maximal velocity for the forward reaction is V1 = .203 pmol/min. The enzyme was inhibited by progesterone, oestradiol-17 beta, cortisol and pharmaceutical drugs. An activating effect could be demonstrated with Ca2+ and oxytocin. The occurrence of PGE2-9-OR in the decidua vera suggests that this enzyme may be responsible for the transformation of PGE2 to PGF2 alpha in these tissues. This may be an important mechanism for the initiation and maintenance of uterine contractions.
前列腺素E2 - 9 -氧化还原酶（PGE2 - 9 - OR），即一种将前列腺素E2（PGE2）转化为前列腺素F2α（PGF2α）的酶，已在人真蜕膜中被检测到。当离心后的匀浆依次通过DEAE - Trisacryl、羟基磷灰石 - 琼脂糖凝胶、Ultrogel AcA 44和Matrex凝胶蓝A凝胶色谱法进行分级分离时，实现了105倍的纯化。已获得PGE2 - 9 - OR的以下动力学常数。关于PGE2的平衡常数为83微摩尔，PGE2的米氏常数Km为80微摩尔，NADPH的米氏常数为1.6微摩尔。正向反应的最大速度为V1 = 0.203皮摩尔/分钟。该酶受到孕酮、雌二醇 - 17β、皮质醇和药物的抑制。Ca2 +和催产素可表现出激活作用。PGE2 - 9 - OR在真蜕膜中的存在表明，该酶可能负责这些组织中PGE2向PGF2α的转化。这可能是子宫收缩启动和维持的一个重要机制。
