Miyauchi F, Kato H, Yamashita H, Ueda K, Tamura H, Mano T, Torigoe T
J Endocrinol. 1984 Jun;101(3):285-8. doi: 10.1677/joe.0.1010285.
The effects of a conceptus-derived substance on the activity of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 20 alpha-HSD in the ovary were studied in the rat. On day 7 of pregnancy (day 1 = insemination), rats were laparotomized and the desired number of conceptuses was aspirated from the uterus; thus, rats carrying one, two, three, four, five to seven or eight to ten conceptuses were prepared. They were autopsied on day 15 and 3 beta-HSD and 20 alpha-HSD activity in the corpus luteum (CL) or non-luteal ovarian tissue ( NLO ) was determined. Conceptus number was directly related to 3 beta-HSD and inversely related to 20 alpha-HSD activity in the CL. The serum progesterone level and CL weight were also directly related to conceptus number. Neither 3 beta-HSD nor 20 alpha-HSD activity in the NLO was affected by conceptus number. These results indicated that 3 beta-HSD and 20 alpha-HSD in the CL are probably regulated by placental hormone secreted in proportion to the number of conceptuses; in the NLO these enzymes may be controlled by a different mechanism.
在大鼠中研究了源自孕体的一种物质对卵巢中3β-羟基类固醇脱氢酶（3β-HSD）和20α-羟基类固醇脱氢酶（20α-HSD）活性的影响。在妊娠第7天（第1天=受精），对大鼠进行剖腹手术，并从子宫中吸出所需数量的孕体；由此，制备了怀有1个、2个、3个、4个、5至7个或8至10个孕体的大鼠。在第15天对它们进行解剖，并测定黄体（CL）或非黄体卵巢组织（NLO）中的3β-HSD和20α-HSD活性。孕体数量与CL中的3β-HSD直接相关，与20α-HSD活性呈负相关。血清孕酮水平和CL重量也与孕体数量直接相关。NLO中的3β-HSD和20α-HSD活性均不受孕体数量的影响。这些结果表明，CL中的3β-HSD和20α-HSD可能受与孕体数量成比例分泌的胎盘激素调节；在NLO中，这些酶可能受不同机制控制。
