Codde J P, Barden A, Vandongen R, Beilin L J
Prostaglandins Leukot Med. 1984 Apr;14(1):55-64. doi: 10.1016/0262-1746(84)90024-6.
Results obtained from radioimmunoassay (RIA) at various stages of sample processing were compared in order to determine the degree of purification necessary to estimate levels of prostaglandins (PG) and thromboxane B2 (TXB2). In serum, TXB2 levels were closely comparable when assayed directly or after organic extraction and thin layer chromatography (TLC). Although concentrations of 6-keto-PGF1 determined by both methods were correlated, levels were significantly overestimated by the direct method. PGE2 and 6-keto-PGF1 alpha levels measured directly in acetone extracted kidney homogenate were almost identical with kidney homogenates taken through the full extraction procedure and TLC. After indomethacin treatment serum 6-keto-PGF1 alpha and PG levels in kidney homogenate assayed directly were markedly different from extracted values. Thus direct RIA of samples is reliable when prostanoid levels are high in relation to interfering substances, as with serum TXB2 and kidney homogenate 6-keto-PGF1 alpha and PGE2, but not for serum 6-keto-PGF1 alpha or following PG inhibition.
为了确定估计前列腺素（PG）和血栓素B2（TXB2）水平所需的纯化程度，对在样品处理的各个阶段通过放射免疫测定（RIA）获得的结果进行了比较。在血清中，直接测定或经过有机萃取和薄层色谱法（TLC）后测定的TXB2水平密切可比。虽然两种方法测定的6-酮-PGF1浓度具有相关性，但直接法显著高估了其水平。在丙酮萃取的肾脏匀浆中直接测定的PGE2和6-酮-PGF1α水平与经过完整萃取程序和TLC处理的肾脏匀浆几乎相同。吲哚美辛治疗后，直接测定的血清6-酮-PGF1α和肾脏匀浆中的PG水平与萃取值明显不同。因此，当类前列腺素水平相对于干扰物质较高时，如血清TXB2以及肾脏匀浆中的6-酮-PGF1α和PGE2，对样品进行直接RIA是可靠的，但对于血清6-酮-PGF1α或在PG抑制后则不可靠。
