Odegård O R, Lie M
Haemostasis. 1978;7(2-3):121-6. doi: 10.1159/000214248.
Methodological problems encountered using chromogenic substrates on thrombin and Xa are discussed: (1) influence of substrate on inhibitor; (2) influence of heparin; (3) optimal concentrations; (4) specificity; (5) denatured enzymes, and (6) natural versus chromogenic substrates.
讨论了使用凝血酶和Xa的显色底物时遇到的方法学问题:(1)底物对抑制剂的影响;(2)肝素的影响;(3)最佳浓度;(4)特异性;(5)变性酶,以及(6)天然底物与显色底物。