Elliott M S, Katze J R, Trewyn R W
Cancer Res. 1984 Aug;44(8):3215-9.
With normal human skin fibroblasts in culture, a transient decrease in queuine modification of tRNA precedes a phorbol ester tumor promoter-induced 5- to 10-fold increase in saturation density. Subsequently, an increase in the queuine content of cellular tRNA (to levels comparable to those in untreated cultures) precedes a decrease in saturation density. This reversal of the phorbol ester-induced alteration in tRNA modification occurs in the continued presence of the tumor promoter, and it parallels an increased ability of the cells to salvage queuine from catabolized endogenous tRNA. Addition of exogenous queuine concurrently with the tumor promoter at early passage significantly inhibits the increase in saturation density. The results suggest a role for the decrease in queuine modification of tRNA in mediating the phenotypic change induced by the tumor promoter.
在培养的正常人皮肤成纤维细胞中，佛波酯肿瘤启动子诱导饱和密度增加5至10倍之前，tRNA的Q修饰会短暂下降。随后，细胞tRNA的Q含量增加（达到与未处理培养物相当的水平），然后饱和密度下降。这种佛波酯诱导的tRNA修饰改变的逆转发生在肿瘤启动子持续存在的情况下，并且与细胞从分解代谢的内源性tRNA中挽救Q的能力增加平行。在早期传代时与肿瘤启动子同时添加外源性Q可显著抑制饱和密度的增加。结果表明，tRNA的Q修饰减少在介导肿瘤启动子诱导的表型变化中起作用。
