Gimeno M F, Franchi A M, Chaud M, Gonzalez E T, Viggiano M, Gimeno A L
Prostaglandins. 1984 May;27(5):737-52. doi: 10.1016/0090-6980(84)90011-x.
The output of prostaglandins (PGs) E1, E2 and F2 alpha, from ampullary and isthmic portions of sow oviducts isolated during proestrus, estrus and metestrus, was explored. Moreover, in vitro cumulative dose-response curves for the contractile effect of these three PGs, on identical oviductal segments, were constructed. Isthmic preparations from proestrous and metestrous animals released more PGE1 and PGF2 alpha than PGE2 "like material". During estrus, the outputs of PGE1, PGE2 and PGF2 alpha were similar, whereas, oviducts from proestrous and metestrous sows released less PGE1 and PGF2 alpha than during estrus. Although the output of PGE2 "like material" from isthmic and ampullary segments did not differ significantly during the three stages of the sex cycle, ampullary metestrous preparations released more PGE1 and PGF2 alpha, than estrous or proestrous ones. The addition of PGE1, PGE2 or PGF2 alpha, consistently stimulated the amplitude of contractions of isthmic oviductal segments isolated from proestrous and metestrous sows. Within the concentration-range explored, dose-response curves for PGE2 and PGE1 were to the left of those for PGF2 alpha in the isthmus obtained before ovulation (proestrus) but not in segments isolated at later times (2-3 days) of the cycle (metestrus). The stimulatory dose-response curves for PGE1, or PGE2, in isthmic segments of metestrous preparations incubated with phentolamine (10(-6)M) were shifted to the right of controls not exposed to the adrenoreceptor blocker, whereas, the curve for PGF2 alpha without phentolamine, was identical to that obtained in its presence. PGE1 and PGE2 did not evoke significant contractile effects on oviductal ampullary portions from proestrous sows, whereas, PGF2 alpha was clearly stimulatory at concentrations of 10(-9)M and higher. In ampullary segments isolated after ovulation (metestrus) the threshold for contractile enhancement following PGF2 alpha was greater than during proestrus, whereas, PGE1 elicited a significant inhibition of contractions. The spontaneous contractile pattern exhibited by isthmic and ampullary oviductal regions, prior to and after ovulation, is discussed in terms of tissue PG generation and output and is compared with results regarding tubal motility following the exposure to exogenous PGs.
研究了在发情前期、发情期和发情后期分离的母猪输卵管壶腹部和峡部前列腺素（PGs）E1、E2和F2α的分泌情况。此外，还构建了这三种PGs对相同输卵管段收缩作用的体外累积剂量反应曲线。发情前期和发情后期动物的峡部制剂释放的PGE1和PGF2α比PGE2“类物质”更多。在发情期，PGE1、PGE2和PGF2α的分泌量相似，而发情前期和发情后期母猪的输卵管释放的PGE1和PGF2α比发情期少。尽管在性周期的三个阶段，峡部和壶腹部段的PGE2“类物质”分泌量没有显著差异，但发情后期壶腹部制剂释放的PGE1和PGF2α比发情期或发情前期的多。添加PGE1、PGE2或PGF2α可持续刺激发情前期和发情后期母猪分离的峡部输卵管段的收缩幅度。在探索的浓度范围内，排卵前（发情前期）峡部中PGE2和PGE1的剂量反应曲线在PGF2α的左侧，但在周期后期（2 - 3天）分离的段（发情后期）中并非如此。用酚妥拉明（10(-6)M）孵育的发情后期制剂峡部段中，PGE1或PGE2的刺激剂量反应曲线相对于未暴露于肾上腺素能受体阻滞剂的对照组向右移动，而没有酚妥拉明时PGF2α的曲线与其存在时获得的曲线相同。PGE1和PGE2对发情前期母猪的输卵管壶腹部部分没有引起显著的收缩作用，而PGF2α在浓度为10(-9)M及更高时明显具有刺激作用。在排卵后（发情后期）分离的壶腹部段中，PGF2α引起收缩增强的阈值高于发情前期，而PGE1引起了显著的收缩抑制。根据组织PG的产生和分泌情况，讨论了排卵前后峡部和壶腹部输卵管区域的自发收缩模式，并与暴露于外源性PGs后输卵管运动的结果进行了比较。
