Dráber P, Stanley P
Somat Cell Mol Genet. 1984 Sep;10(5):445-54. doi: 10.1007/BF01534849.
Cytotoxic plant lectins have been used for the single-step selection of mouse embryonal carcinoma cell mutants with altered expression of surface glycoconjugates. Following mutagenesis, several F9 and OTF9-63 cell lines resistant to the lectins from Triticum vulgaris or Ricinus communis were obtained. At least five distinct lectin-resistant (LecR) phenotypes have been identified on the basis of their relative sensitivities to four different plant lectins and their altered lectin-binding properties. None of the mutant types exhibits a significant change in the ability to bind a monoclonal antibody against the stage-specific embryonic antigen, SSEA-1. All of the mutants form aggregates when cultured in bacteriological petri dishes and appear to differentiate into endoderm-like cells following exposure to retinoic acid. However, two of the LecR cell lines exhibit an altered morphology when grown on a plastic substratum.
细胞毒性植物凝集素已被用于单步筛选表面糖缀合物表达改变的小鼠胚胎癌细胞突变体。诱变后，获得了几种对普通小麦或蓖麻凝集素具有抗性的F9和OTF9-63细胞系。根据它们对四种不同植物凝集素的相对敏感性及其改变的凝集素结合特性，至少鉴定出五种不同的凝集素抗性（LecR）表型。没有一种突变类型在结合针对阶段特异性胚胎抗原SSEA-1的单克隆抗体的能力上表现出显著变化。所有突变体在细菌培养皿中培养时都会形成聚集体，并且在暴露于视黄酸后似乎会分化为内胚层样细胞。然而，其中两个LecR细胞系在塑料基质上生长时表现出形态改变。
