Daar A S, Fuggle S V, Fabre J W, Ting A, Morris P J
Transplantation. 1984 Sep;38(3):293-8. doi: 10.1097/00007890-198409000-00019.
In a previous article we described the detailed tissue distribution of MHC class I antigens. In this study, we have used a monoclonal antibody, NFK1, to study the tissue distribution of MHC class II antigens. This antibody, which detects a monomorphic determinant common to the DR, SB, and DC molecules, was used to stain frozen sections of normal tissues from throughout the human body by a sensitive peroxidase-antiperoxidase immunohistological technique. Although previous studies, both in animal models and in human beings, have shown that class II antigens are expressed on a limited number of nonlymphoid tissues, our study has extended the spectrum of tissues on which this class of antigens is detectable. Epithelial cells in a number of organs were positively stained--these include the tongue, tonsils, epiglottis, trachea, small intestine, urethra, epididymis, and proximal renal tubules. Lymphatics throughout the body appeared to express class II antigens. Capillaries in brain, testis, and placenta appeared not to express class II antigens, but in the rest of the body they showed strong and uniform staining. These and other observations, and their implications, are discussed in relation to previously published studies.
在之前的一篇文章中，我们描述了MHC I类抗原详细的组织分布情况。在本研究中，我们使用一种单克隆抗体NFK1来研究MHC II类抗原的组织分布。该抗体可检测DR、SB和DC分子共有的一个单态决定簇，采用灵敏的过氧化物酶-抗过氧化物酶免疫组织学技术，用其对来自人体各处的正常组织冰冻切片进行染色。尽管之前在动物模型和人体中的研究均表明II类抗原仅在有限数量的非淋巴组织中表达，但我们的研究扩展了可检测到这类抗原的组织范围。许多器官中的上皮细胞呈阳性染色，这些器官包括舌、扁桃体、会厌、气管、小肠、尿道、附睾和近端肾小管。全身的淋巴管似乎都表达II类抗原。脑、睾丸和胎盘的毛细血管似乎不表达II类抗原，但在身体其他部位它们呈现出强烈且均匀的染色。结合之前发表的研究对这些及其他观察结果及其意义进行了讨论。
