Tsuji M, Takeyama M, Takatsuka D, Yabumoto H, Terada N, Matsumoto K
J Steroid Biochem. 1984 Aug;21(2):179-83. doi: 10.1016/0022-4731(84)90381-9.
Homogenates of the epididymis, testis and seminal vesicle from 15, 25, 35 and 60-day old golden hamsters were incubated with [3H]4-androstene-3,17-dione and NADPH and enzyme activity was estimated. In the testis, activities of 5 alpha- and 5 beta-reductases were the highest (32 +/- 4 and 68 +/- 13 (SD) nmol/100 mg protein/h, respectively) at 25 days of age, moderately high at 35 days and very low (1-3 and 5-6, nmol/100 mg protein/h, respectively) at 15 and 60 days. In the epididymis, 5 alpha-reductase activities increased with age during sexual development by up to 50-fold and reached the maximum value (40 +/- 11 nmol/100 mg protein/h) at 60 days. The 5 alpha-reductase activities in the seminal vesicle were found to be relatively low (0.5-1 nmol/100 mg protein/h), compared with those in the epididymis and testis. Activities of 5 beta-reductase in the epididymis and seminal vesicle were very low (0.1-0.5 nmol/100 mg protein/h) at all ages. These results indicate the existence of a marked, but transient, increase in 5 alpha- and 5 beta-reductase activities during immature period in the testis of golden hamster. In the epididymis, however, 5 alpha-reductase activity increases with age during sexual maturation, while 5 beta-reductase activity is almost undetectable at all ages.
将15日龄、25日龄、35日龄和60日龄金黄仓鼠的附睾、睾丸和精囊匀浆与[3H]4-雄烯-3,17-二酮及烟酰胺腺嘌呤二核苷酸磷酸（NADPH）一起孵育，并测定酶活性。在睾丸中，5α-还原酶和5β-还原酶的活性在25日龄时最高（分别为32±4和68±13（标准差）nmol/100mg蛋白质/小时），35日龄时中等偏高，15日龄和60日龄时非常低（分别为1 - 3和5 - 6 nmol/100mg蛋白质/小时）。在附睾中，5α-还原酶活性在性发育过程中随年龄增长增加至50倍，并在60日龄时达到最大值（40±11 nmol/100mg蛋白质/小时）。与附睾和睾丸中的5α-还原酶活性相比，精囊中该酶活性相对较低（0.5 - 1 nmol/100mg蛋白质/小时）。附睾和精囊中5β-还原酶的活性在所有年龄阶段都非常低（0.1 - 0.5 nmol/100mg蛋白质/小时）。这些结果表明，金黄仓鼠睾丸在未成熟时期，5α-还原酶和5β-还原酶活性存在显著但短暂的增加。然而，在附睾中，5α-还原酶活性在性成熟过程中随年龄增长而增加，而5β-还原酶活性在所有年龄几乎都检测不到。
