紫外线诱发豚鼠皮肤角质形成细胞不同分化阶段的前列腺素生物合成。

Ultraviolet-evoked prostaglandin biosynthesis in varying stages of keratinocyte differentiation in guinea pig skin.

作者信息

Karmali R A, Safai B

出版信息

Prostaglandins Leukot Med. 1984 Sep;15(3):277-86. doi: 10.1016/0262-1746(84)90127-6.

Abstract

Prostaglandin (PG) production by guinea pig epidermal cells was evaluated at various incubation intervals in normal and UV-exposed cultures. Prostaglandins have been implicated as mediators of the early phase of erythema in skin exposed to sunlight or UV-radiation. Using a density gradient centrifugation procedure, the epidermal cells were fractionated according to the various maturation stages of epidermal keratinocytes: high-density epidermal cells (HDEC) consisting of round, less mature cells; low-density epidermal cells (LDEC) consisting of polygonal keratinized cells; and intermediate-density epidermal cells (IDEC) consisting of both HDEC and LDEC. When cultures of 1 X 10(6) cells were incubated at 37 degrees C in 5% CO2 the highest concentrations of five PG moieties measured were present in supernatants from the LDEC cultures as compared to those of IDEC or HDEC. Levels of PGF 2 alpha were much higher than the rest, which were found in the order PGF2 alpha greater than PGE2 greater than PGE1 greater than 6-keto-PGF1 alpha greater than thromboxane (TX)B2. UV-irradiation induced increases in all but TXA2 production. These results identify and quantitate five compounds produced as a result of exaggerated activity of the cyclooxygenase induced by UV-irradiation.

摘要

在正常培养和紫外线照射的培养物中，于不同孵育时间评估豚鼠表皮细胞的前列腺素（PG）生成情况。前列腺素被认为是皮肤暴露于阳光或紫外线辐射后红斑早期阶段的介质。采用密度梯度离心法，根据表皮角质形成细胞的不同成熟阶段对表皮细胞进行分级：高密度表皮细胞（HDEC）由圆形、不太成熟的细胞组成；低密度表皮细胞（LDEC）由多边形角质化细胞组成；中密度表皮细胞（IDEC）由HDEC和LDEC两者组成。当将1×10⁶个细胞的培养物在37℃、5%二氧化碳条件下孵育时，与IDEC或HDEC培养物的上清液相比，LDEC培养物上清液中所测的五种PG部分的浓度最高。前列腺素F2α的水平远高于其他几种，其顺序为前列腺素F2α＞前列腺素E2＞前列腺素E1＞6 - 酮 - 前列腺素F1α＞血栓素（TX）B2。除血栓素A2生成外，紫外线照射可诱导其他所有物质生成增加。这些结果鉴定并定量了因紫外线照射诱导的环氧化酶活性增强而产生的五种化合物。