Analysis of RNA synthesized in isolated nuclei of Ehrlich ascites tumor cells.
作者信息
Nakanishi Y, Mizuno D, Natori S
出版信息
J Biochem. 1978 Apr;83(4):989-94. doi: 10.1093/oxfordjournals.jbchem.a132027.
Abstract
The molecular size and poly-A content of RNA synthesized in isolated nuclei of Ehrlich ascites tumor cells were measured. KCl was found to be essential for synthesis of high molecular weight RNA: when 0.4 M KCl was added to the reaction mixture, the average molecular size of the RNA formed was 14S; without KCl the average molecular size was 5S. A significant amount of poly-A sequences was found in RNA synthesized in the presence of alpha-amanitin, suggesting that RNA polymerase I and/or III may synthesized some RNA containing poly-A in isolated nuclei.
摘要
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