Graham E E
Arch Oral Biol. 1984;29(10):821-5. doi: 10.1016/0003-9969(84)90012-8.
Little is known about protein biosynthesis during enameloid formation. The purpose of this study was to characterize protein biosynthesis during selachian amelogenesis. Experiments were conducted on intact shark tooth organs, surgically removed from the spiny dogfish, Squalus acanthias. These organs were cultured in medium labelled with [35S]-methionine during time periods ranging from 1 to 6 h in pulse/chase experiments. The proteins were extracted at the conclusion of the experiments for analysis by gel electrophoresis and fluorography. In addition, tooth organs were fixed and examined by light microscopic autoradiography. Through this outlined experimental strategy, protein biosynthesis during amelogenesis was examined. The findings suggest the ameloblasts, at early bell stage of tooth development, are biosynthetically more active than the incipient odontoblasts; and they are secreting two polypeptides, 55 and 43 kd, into the developing tooth extracellular matrix.
关于釉质形成过程中的蛋白质生物合成，人们了解甚少。本研究的目的是描述板鳃亚纲动物釉质发生过程中的蛋白质生物合成。实验采用完整的鲨鱼牙齿器官，这些器官是从棘鲨（Squalus acanthias）身上手术摘除的。在脉冲/追踪实验中，将这些器官在标记有[35S]-甲硫氨酸的培养基中培养1至6小时。实验结束时提取蛋白质，通过凝胶电泳和荧光自显影进行分析。此外，对牙齿器官进行固定，并通过光学显微镜放射自显影进行检查。通过这一概述的实验策略，研究了釉质发生过程中的蛋白质生物合成。研究结果表明，在牙齿发育的早期钟状期，成釉细胞的生物合成活性比初期的成牙本质细胞更高；并且它们正在向发育中的牙齿细胞外基质分泌两种多肽，分子量分别为55和43千道尔顿。
