Zeitlin H C, Weatherall D J
Mol Biol Med. 1983 Dec;1(5):489-500.
Human chromosome 16, which contains the alpha globin gene complex, has been introduced into mouse erythroleukaemia cells by means of cell fusion and selectively retained to the exclusion of other human chromosomes. After induction of haemoglobin synthesis in hybrid clones, evidence for expression of the human alpha globin genes was sought by mRNA and globin chain synthesis analyses. It was found that both the human alpha 1 and alpha 2 genes were similarly expressed and that the synthesis of human alpha globin chains was nearly half that of mouse alpha chains on a per gene basis. The pattern of human alpha gene expression was similar with different inducers and with donor chromosomes of either erythroid or non-erythroid origin. However, the closely linked human embryonic alpha gene (zeta) did not produce detectable levels of zeta globin mRNA in any of the hybrid clones. Thus, there is selective activation of adult genes in the human alpha globin gene complex in the mouse erythroleukaemia cell.
包含α珠蛋白基因复合体的人类16号染色体，已通过细胞融合的方式导入小鼠红白血病细胞，并被选择性保留，而排除了其他人类染色体。在杂交克隆中诱导血红蛋白合成后，通过mRNA和珠蛋白链合成分析来寻找人类α珠蛋白基因表达的证据。结果发现，人类α1和α2基因的表达相似，并且在每个基因的基础上，人类α珠蛋白链的合成量几乎是小鼠α链的一半。不同诱导剂以及来源于红系或非红系的供体染色体，人类α基因的表达模式相似。然而，紧密连锁的人类胚胎α基因（ζ）在任何杂交克隆中都未产生可检测水平的ζ珠蛋白mRNA。因此，在小鼠红白血病细胞中，人类α珠蛋白基因复合体中的成人基因存在选择性激活。
