Deisseroth A, Bode U, Fontana J, Hendrick D
Nature. 1980 May 1;285(5759):36-8. doi: 10.1038/285036a0.
We have developed a system which can be used to study the mechanisms that may govern the expression of human alpha-globin genes in human erythroid and non-erythroid haematopoietic cells. Human chromosome 16, which has been shown to bear the human alpha-globin genes, is introduced by cell fusion into mouse erythroleukemia (MEL) cells to generate continuously proliferating cell lines that retain permanently the human alpha-globin genes. We have shown that hybrid diploid MEL cells with human alpha-globin genes from erythroid donor cells express these genes fully through globin chain synthesis, while hybrid diploid MEL cells containing human alpha-globin genes from non-erythroid human haematopoietic donor cells contain very low levels of human alpha-globin mRNA and no detectable human alpha-globin chains. The levels of human alpha-globin mRNA in these hybrid cells were found to depend on factors present in the MEL recipient cell as well as on the differentiated state of the human donor cell, suggesting that this system may be suitable for characterisation of mechanisms governing haematopoietic differentiation in man.
我们开发了一种系统，可用于研究可能调控人类α-珠蛋白基因在人类红系和非红系造血细胞中表达的机制。已证明携带人类α-珠蛋白基因的人类16号染色体通过细胞融合被引入小鼠红白血病（MEL）细胞，以产生持续增殖的细胞系，这些细胞系永久性保留人类α-珠蛋白基因。我们已经表明，来自红系供体细胞的携带人类α-珠蛋白基因的杂交二倍体MEL细胞通过珠蛋白链合成充分表达这些基因，而含有来自非红系人类造血供体细胞的人类α-珠蛋白基因的杂交二倍体MEL细胞含有极低水平的人类α-珠蛋白mRNA，且未检测到人类α-珠蛋白链。发现这些杂交细胞中人类α-珠蛋白mRNA的水平取决于MEL受体细胞中存在的因子以及人类供体细胞的分化状态，这表明该系统可能适用于表征调控人类造血分化的机制。
