Yeoh G C, Greenstein D, Holtzer H
J Cell Biol. 1978 Apr;77(1):99-102. doi: 10.1083/jcb.77.1.99.
Nuclei within myotubes do not synthesize DNA for replication. Accordingly, cultures of myotubes display low levels of DNA polymerase activity. The coincidental decline in DNA polymerase activity and increased formation of multinucleated myotubes during culture does not prove that the loss of capacity to synthesize DNA is a consequence of fusion. Tne experiments described demonstrate that myogenic cells prevented from fusing have low levels of DNA polymerase activity. This is consistent with the notion that, in myogenic cultures, there is a population of mononucleated cells, the myoblasts, which have withdrawn from the mitotic cycle before fusion.
肌管内的细胞核不会合成用于复制的DNA。因此，肌管培养物显示出低水平的DNA聚合酶活性。培养过程中DNA聚合酶活性的同时下降以及多核肌管形成的增加，并不能证明合成DNA能力的丧失是融合的结果。所描述的实验表明，被阻止融合的成肌细胞具有低水平的DNA聚合酶活性。这与以下观点一致：在成肌培养物中，存在一群单核细胞，即成肌细胞，它们在融合前已退出有丝分裂周期。
