Yanagi S, Tsuda H, Sakamoto M, Ninomiya Y, Ito N
J Natl Cancer Inst. 1984 Dec;73(6):1311-7.
The histologic classification of mouse liver tumors, i.e., hepatic nodules type 1 and type 2 and hepatocellular carcinomas, was evaluated by a comparison of several biologic and biochemical markers that have been shown to be useful for the grading of tumor malignancy. The liver tumors were induced by N,N'-2,7-fluorenylenebisacetamide (CAS: 304-28-9; N,N'-fluoren-2,7-ylenebisacetamide) administration to male CD-1 mice. The ability to induce L-type pyruvate kinase activity in response to a high-carbohydrate diet disappeared in almost all the liver tumors. However, fairly good correlations were observed between the histologic classification and the relative weights of liver and intraperitoneal fat pads, between the histologic classification and the level of serum total cholesterol, and between the histologic classification and the K-type pyruvate kinase activity. The results suggest that the present histologic classification reflects the degree of tumor malignancy, and therefore, it would be useful for the classification of mouse liver tumors.
通过比较几种已被证明对肿瘤恶性程度分级有用的生物学和生化标志物，对小鼠肝肿瘤的组织学分类，即1型和2型肝结节以及肝细胞癌进行了评估。肝肿瘤是通过向雄性CD-1小鼠施用N，N'-2，7-芴亚基双乙酰胺（CAS：304-28-9；N，N'-芴-2，7-亚基双乙酰胺）诱导产生的。几乎所有肝肿瘤对高碳水化合物饮食产生反应诱导L型丙酮酸激酶活性的能力都消失了。然而，在组织学分类与肝脏和腹膜内脂肪垫的相对重量之间、组织学分类与血清总胆固醇水平之间以及组织学分类与K型丙酮酸激酶活性之间观察到了相当好的相关性。结果表明，目前的组织学分类反映了肿瘤的恶性程度，因此，它将有助于小鼠肝肿瘤的分类。
